Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins

Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins

SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4...

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Main Author: Aleksander F. Sikorski
Format: Article
Language:English
Published: Polish Botanical Society 2015-01-01
Series:Acta Societatis Botanicorum Poloniae
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/4810
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spelling doaj-5719299a78ce430184b2314084854db72020-11-25T03:11:14ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-01-0148451152110.5586/asbp.1979.0424013Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteinsAleksander F. Sikorski0University of WrocławSDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides.https://pbsociety.org.pl/journals/index.php/asbp/article/view/4810
collection DOAJ
language English
format Article
sources DOAJ
author Aleksander F. Sikorski
spellingShingle Aleksander F. Sikorski
Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
Acta Societatis Botanicorum Poloniae
author_facet Aleksander F. Sikorski
author_sort Aleksander F. Sikorski
title Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
title_short Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
title_full Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
title_fullStr Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
title_full_unstemmed Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins
title_sort electrophoretic analysis, labeling and isolation of chlamydomonas reinhardtii flagellum membrane proteins
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2015-01-01
description SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides.
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/4810
work_keys_str_mv AT aleksanderfsikorski electrophoreticanalysislabelingandisolationofchlamydomonasreinhardtiiflagellummembraneproteins
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