| Summary: | Capturing high spatial resolution imagery is becoming a standard operation in many agricultural applications. The increased capacity for image capture necessitates corresponding advances in analysis algorithms. This study introduces automated raster geoprocessing methods to automatically extract strawberry (<i>Fragaria</i> × <i>ananassa</i>) canopy size metrics using raster image analysis and utilize the extracted metrics in statistical modeling of strawberry dry weight. Automated canopy delineation and canopy size metrics extraction models were developed and implemented using ArcMap software v 10.7 and made available by the authors. The workflows were demonstrated using high spatial resolution (1 mm resolution) orthoimages and digital surface models (2 mm) of 34 strawberry plots (each containing 17 different plant genotypes) planted on raised beds. The images were captured on a weekly basis throughout the strawberry growing season (16 weeks) between early November and late February. The results of extracting four canopy size metrics (area, volume, average height, and height standard deviation) using automatically delineated and visually interpreted canopies were compared. The trends observed in the differences between canopy metrics extracted using the automatically delineated and visually interpreted canopies showed no significant differences. The R<sup>2</sup> values of the models were 0.77 and 0.76 for the two datasets and the leave-one-out (LOO) cross validation root mean square error (RMSE) of the two models were 9.2 g and 9.4 g, respectively. The results show the feasibility of using automated methods for canopy delineation and canopy metric extraction to support plant phenotyping applications.
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