An improved method for extraction of microbial DNA from alkaline-saline soil

An improved method for extraction of microbial DNA from alkaline-saline soil

A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (Mo Bio™ Laboratories, Inc.) was used as a ref...

Full description

Bibliographic Details
Main Authors:	Valentín Pérez-Hernández, Mario Hernández-Guzmán, César Valenzuela-Encinas, Rocío Alcántara-Hernández, Isabel Estrada-Alvarado, Luc Dendooven, Rodolfo Marsch, Federico Gutiérrez-Miceli, Víctor M. Ruíz-Valdiviezo, Joaquín A. Montes-Molina
Format:	Article
Language:	English
Published:	Sociedad Mexicana de la Ciencia del Suelo A. C. 2021-06-01
Series:	Terra Latinoamericana
Subjects:	dna extraction dna sequencing pcr amplification saline-alkaline soil
Online Access:	https://www.terralatinoamericana.org.mx/index.php/terra/article/view/887

Similar Items

Germination, Growth, Chlorophyll Fluorescence and Ionic Balance in Linseed Seedlings Subjected to Saline and Alkaline Stresses
by: Rui Guo, et al.
Published: (2014-01-01)

Biocontrol potential of saline- or alkaline-tolerant Trichoderma asperellum mutants against three pathogenic fungi under saline or alkaline stress conditions
by: Ruiting Guo, et al.

Screening and Evaluation of Saline–Alkaline Tolerant Germplasm of Rice (Oryza sativa L.) in Soda Saline–Alkali Soil
by: Hao Wang, et al.
Published: (2018-09-01)

Optimal Design of Alkaline–Surfactant–Polymer Flooding under Low Salinity Environment
by: Adi Novriansyah, et al.
Published: (2020-03-01)

Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat
by: Huanan Han, et al.
Published: (2018-09-01)

Elementary sulfur or calcium sulfate to the remediation of saline-sodic soils?
by: Josinaldo Lopes Araujo, et al.
Published: (2015-12-01)

Nonionic Surfactant to Enhance the Performances of Alkaline–Surfactant–Polymer Flooding with a Low Salinity Constraint
by: Shabrina Sri Riswati, et al.
Published: (2020-05-01)

Research progress on plant tolerance to soil salinity and alkalinity in sorghum
by: Rui-dong HUANG
Published: (2018-04-01)

SALINE SOILS IN THE EASTERN PART OF THE YERGENES
by: A. Novikova, et al.
Published: (2011-06-01)

Mapping Soil Alkalinity and Salinity in Northern Songnen Plain, China with the HJ-1 Hyperspectral Imager Data and Partial Least Squares Regression
by: Lin Bai, et al.
Published: (2018-11-01)

The Adjustment of Membrane Lipid Metabolism Pathways in Maize Roots Under Saline–Alkaline Stress
by: Xiaoxuan Xu, et al.
Published: (2021-03-01)

Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice
by: Kim, Y.-A, et al.
Published: (2022)

Saline and Alkaline Tolerance of Wetland Plants—What are the Most Representative Evaluation Indicators?
by: Rui Cheng, et al.
Published: (2020-03-01)

Application of compound material alleviates saline and alkaline stress in cotton leaves through regulation of the transcriptome
by: Mengjie An, et al.
Published: (2020-10-01)

Ca<sup>2+</sup>/Na<sup>+</sup> Ratio as a Critical Marker for Field Evaluation of Saline-Alkaline Tolerance in Alfalfa (<i>Medicago sativa</i> L.)
by: Tian-Jiao Wei, et al.
Published: (2020-01-01)

Effects of mixed saline and alkaline stress on the morphology and anatomy of Pisum sativum L.: The role of peroxidase and ascorbate oxidase in growth regulation
by: Kukavica Biljana, et al.
Published: (2013-01-01)

Remote Sensing of Soil Alkalinity and Salinity in the Wuyu’er-Shuangyang River Basin, Northeast China
by: Lin Bai, et al.
Published: (2016-02-01)

Effects of intracuff alkalinized lignocaine, dexamethasone, or normal saline on endotracheal tube-induced emergence phenomena: A randomized double-blinded study
by: Teena Desai, et al.
Published: (2019-01-01)

High‐Performance Ammonium Cobalt Phosphate Nanosheet Electrocatalyst for Alkaline Saline Water Oxidation
by: Zhongxin Song, et al.
Published: (2021-07-01)

Procedures for DNA extraction and amplification from quills of crested porcupine (<em>Hystrix cristata</em>)
by: Domenico Fulgione
Published: (2000-09-01)

Enhancing PCR Amplification of DNA from Recalcitrant Plant Specimens Using a Trehalose-Based Additive
by: Tharangamala Samarakoon, et al.
Published: (2013-01-01)

Application of genetic and evolutionary approaches in marine conservation and management (Case study: PANGAS project)
by: TOIC Katarina
Published: (2016-12-01)

Sulfate reduction controlled by organic matter availability in deep sediment cores from the saline, alkaline Lake Van (Eastern Anatolia, Turkey)
by: Clemens eGlombitza, et al.
Published: (2013-07-01)

Label-free DNA detection by loop- mediated isothermal amplification coupled with quartz crystal microbalance sensor
by: Watcharee Boonlue, et al.
Published: (2014-12-01)

Effects of nitrogen application rate and hill density on rice yield and nitrogen utilization in sodic saline–alkaline paddy fields
by: Xiao-hong GUO, et al.
Published: (2021-02-01)

Effects of Organic Polymer Compound Material on K+ and Na+ Distribution and Physiological Characteristics of Cotton Under Saline and Alkaline Stresses
by: Xiaoli Wang, et al.
Published: (2021-05-01)

Electricity Generation, Salt and Nitrogen Removal and Microbial Community in Aircathode Microbial Desalination Cell for Saline-Alkaline Soil-Washing Water Treatment
by: Chenglong Xu, et al.
Published: (2020-08-01)

Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress
by: Ruoyi Lin, et al.
Published: (2021-07-01)

Arbuscular mycorrhizal fungi (AMF) species and abundance exhibit different effects on saline-alkaline tolerance in Leymus chinensis
by: Yaping Cao, et al.
Published: (2020-01-01)

A Novel Method of Genomic DNA Extraction for Cactaceae
by: Shannon D. Fehlberg, et al.
Published: (2013-03-01)

“Two‐steps” seed‐derived plugs as an effective propagation method for the establishment of Miscanthus in saline–alkaline soil
by: Cheng Zheng, et al.
Published: (2021-06-01)

Fitness Cost of Transgenic cry1Ab/c Rice Under Saline-Alkaline Soil Condition
by: Jianmei Fu, et al.
Published: (2018-10-01)

Comparative transcriptome analysis reveals gene expression differences between two peach cultivars under saline-alkaline stress
by: Shuxia Sun, et al.
Published: (2020-03-01)

A mechanistic basis for amplification differences between samples and between genome regions
by: Veal Colin D, et al.
Published: (2012-09-01)

Role of Melatonin in Plant Tolerance to Soil Stressors: Salinity, pH and Heavy Metals
by: Mohamed Moustafa-Farag, et al.
Published: (2020-11-01)

Determination of Magnesium Salinity in Belozem Region, Bulgaria
by: Nataliya Andreeva
Published: (2020-05-01)

Novel Strategies to Optimize the Amplification of Single-Stranded DNA
by: Atef Nehdi, et al.
Published: (2020-05-01)

Effect of Salinity and Compost on Some Enzymes Activity in Rizhosphere of Bean and Tomato Plants
by: Luma S.J.Al-Taweel, et al.
Published: (2016-06-01)

Use of DAF markers (DNA Amplification Fingerprint) to Assess Genetic Diversity of Rice (Oryza satival L.)
by: Baghdad Science Journal
Published: (2009-06-01)

Comparação entre métodos de estocagem de DNA extraído de amostras de sangue, sêmen e pêlos e entre técnicas de extração Comparison between storage methods of DNA extracted from blood, semen and hair and between the techniques of extraction
by: E.G.A. Coelho, et al.
Published: (2004-02-01)