Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design

• Main Authors: Juan Zhong, Xiaoyong Zhang, Yanli Ren, Jie Yang, Hong Tan, Jinyan Zhou
• Format: Article
• Language: English
• Published: Elsevier 2014-05-01
• Series: Electronic Journal of Biotechnology
• Subjects: Bacillus subtilis ZK8; Iturin A; Response surface methodology
• Online Access: http://www.sciencedirect.com/science/article/pii/S0717345814000517

Background: Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor. Results: The results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/L). This led to a 44% increase of JAA production in fed batch fermentation as compared to the production of batch fermentation. Conclusion: The results of this work improve the present production of JAA and may be adopted for other objective products' production.