RA PD-ANALYS IS OF PSEUDOMONAS SYRINGAE, ISOLATED FROM WEEDS IN AGRO PHYTO CENOS IS OF WHEAT

• Main Authors: О. А. Савенко, Л. М. Буценко, Л. А. Пасічник, В. П. Патика
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2014-09-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: pseudomonas syringae; генетична різноманітність; rаpd‑плр аналіз
• Online Access: http://mbt.onu.edu.ua/article/view/48287

Aim. Investigation of the genetic diversity of Pseudomonas syringae strains, isolated from various weeds: horsetail, field bindweed, barnyard grass, sow-thistle, bedstraw, wild radish, orache white whith had the signs of bacterial affection. Methods. RAPD-PGR analysis. Results. Pseudomonas syringae strains, isolated from various species of weeds in wheat agrophytocenosis, were analyzed. We have established the relationship of studied strains with neopatotype strain Pseudomonas syringae pv. atrofaciens PDDCC 4394, and the type strains of Pseudomonas syringae pv. syringae NCPPB 281. Most isolated strains had a high level of affinity with basal bacteriosis pathogen of wheat Pseudomonas syringae pv. atrofaciens which is the most common among the crops. Bacterial blight pathogen Pseudomonas syringae pv. syringae is less widespread and only three bacteria stains isolated from weeds had common reaction. Conclusion. P. syringae strains isolated from different types of weeds and Pseudomonas syringae pv. syringae NCPPB and Pseudomonas syringae pv. atrofaciens PDDCC4394 strains, pathogens of bacterial diseases of wheat are genetically homogeneous group. Our data support the hypothesis that weeds are one of the ecological niches of conservation and survival of bacterial pathogens and under favorable conditions can be a source of infection.