Deconstructing the Polymerase Chain Reaction II: an improved workflow and effects on artifact formation and primer degeneracy

Deconstructing the Polymerase Chain Reaction II: an improved workflow and effects on artifact formation and primer degeneracy

Polymerase chain reaction (PCR) amplification of complex microbial genomic DNA templates with degenerate primers can lead to distortion of the underlying community structure due to inefficient primer-template interactions leading to bias. We previously described a method of deconstructed PCR (“PEX P...

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Bibliographic Details
Main Authors: Ankur Naqib, Silvana Poggi, Stefan J. Green
Format: Article
Language:English
Published: PeerJ Inc. 2019-06-01
Series:PeerJ
Subjects:
PCR
Chimera
PCR bias
Microbiome
Primer variants
Online Access:https://peerj.com/articles/7121.pdf

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