Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice

Ishizuchi dark tea is a microorganism-fermented tea produced in Ehime, Japan. We herein describe the anti-allergic effect of Ishizuchi dark tea. The water extract from Ishizuchi dark tea suppressed degranulation of RBL-2H3 cells in a dose-dependent manner with no apparent cytotoxicity. Fractionation...

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Main Authors:	Michiyo Kondo, Kosuke Nishi, Takuya Sugahara
Format:	Article
Language:	English
Published:	Elsevier 2015-04-01
Series:	Journal of Functional Foods
Subjects:	Microorganism-fermented tea Theabrownin Anti-allergic activity Mast cell degranulation RBL-2H3 cells Mice
Online Access:	http://www.sciencedirect.com/science/article/pii/S1756464615001085

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record_format	Article
spelling	doaj-5661742f50ed475b8a87d040e27786c62021-04-29T04:44:03ZengElsevierJournal of Functional Foods1756-46462015-04-0114659669Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in miceMichiyo Kondo0Kosuke Nishi1Takuya Sugahara2Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, JapanFaculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, JapanFaculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan; Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime 790-8566, Japan; South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime 798-4205, Japan; Corresponding author. Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan. Tel.: +81 89 946 9863; fax: +81 89 946 9863.Ishizuchi dark tea is a microorganism-fermented tea produced in Ehime, Japan. We herein describe the anti-allergic effect of Ishizuchi dark tea. The water extract from Ishizuchi dark tea suppressed degranulation of RBL-2H3 cells in a dose-dependent manner with no apparent cytotoxicity. Fractionation and β-hexosaminidase release assay of the tea components revealed theabrownins as the major bioactive substance in Ishizuchi dark tea. Phosphorylation of Syk, a tyrosine kinase, tended to be suppressed by treating RBL-2H3 cells with theabrownins. In addition, Ishizuchi dark tea suppressed an allergic symptom in the Cry j1-induced pollinosis model mice. Taken together, these data indicated that Ishizuchi dark tea would be a beneficial functional food with an anti-allergic effect.http://www.sciencedirect.com/science/article/pii/S1756464615001085Microorganism-fermented teaTheabrowninAnti-allergic activityMast cell degranulationRBL-2H3 cellsMice
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Michiyo Kondo Kosuke Nishi Takuya Sugahara
spellingShingle	Michiyo Kondo Kosuke Nishi Takuya Sugahara Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice Journal of Functional Foods Microorganism-fermented tea Theabrownin Anti-allergic activity Mast cell degranulation RBL-2H3 cells Mice
author_facet	Michiyo Kondo Kosuke Nishi Takuya Sugahara
author_sort	Michiyo Kondo
title	Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice
title_short	Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice
title_full	Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice
title_fullStr	Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice
title_full_unstemmed	Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice
title_sort	ishizuchi dark tea suppresses ige-mediated degranulation of rbl-2h3 cells and nasal rubbing behavior of pollinosis in mice
publisher	Elsevier
series	Journal of Functional Foods
issn	1756-4646
publishDate	2015-04-01
description	Ishizuchi dark tea is a microorganism-fermented tea produced in Ehime, Japan. We herein describe the anti-allergic effect of Ishizuchi dark tea. The water extract from Ishizuchi dark tea suppressed degranulation of RBL-2H3 cells in a dose-dependent manner with no apparent cytotoxicity. Fractionation and β-hexosaminidase release assay of the tea components revealed theabrownins as the major bioactive substance in Ishizuchi dark tea. Phosphorylation of Syk, a tyrosine kinase, tended to be suppressed by treating RBL-2H3 cells with theabrownins. In addition, Ishizuchi dark tea suppressed an allergic symptom in the Cry j1-induced pollinosis model mice. Taken together, these data indicated that Ishizuchi dark tea would be a beneficial functional food with an anti-allergic effect.
topic	Microorganism-fermented tea Theabrownin Anti-allergic activity Mast cell degranulation RBL-2H3 cells Mice
url	http://www.sciencedirect.com/science/article/pii/S1756464615001085
work_keys_str_mv	AT michiyokondo ishizuchidarkteasuppressesigemediateddegranulationofrbl2h3cellsandnasalrubbingbehaviorofpollinosisinmice AT kosukenishi ishizuchidarkteasuppressesigemediateddegranulationofrbl2h3cellsandnasalrubbingbehaviorofpollinosisinmice AT takuyasugahara ishizuchidarkteasuppressesigemediateddegranulationofrbl2h3cellsandnasalrubbingbehaviorofpollinosisinmice
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Ishizuchi dark tea suppresses IgE-mediated degranulation of RBL-2H3 cells and nasal rubbing behavior of pollinosis in mice

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