A validation scale to determine the readiness of environmental DNA assays for routine species monitoring

A validation scale to determine the readiness of environmental DNA assays for routine species monitoring

Abstract The use of environmental DNA (eDNA) analysis for species monitoring requires rigorous validation—from field sampling to the analysis of PCR‐based results—for meaningful application and interpretation. Assays targeting eDNA released by individual species are typically validated with no prede...

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Bibliographic Details
Main Authors: Bettina Thalinger, Kristy Deiner, Lynsey R. Harper, Helen C. Rees, Rosetta C. Blackman, Daniela Sint, Michael Traugott, Caren S. Goldberg, Kat Bruce
Format: Article
Language:English
Published: Wiley 2021-07-01
Series:Environmental DNA
Subjects:
digital PCR
eDNA
endpoint PCR
https://edna‐validation.com
quantitative PCR
species detection
Online Access:https://doi.org/10.1002/edn3.189

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