Aconitase and Developmental End Points as Early Indicators of

• Main Author: Oleksandr Vasyliovuch Lozinsky
• Format: Article
• Language: English
• Published: Arak University of Medical Sciences 2014-03-01
• Series: Iranian Journal of Toxicology
• Subjects: Aconitate Hydratase; Drosophila Melanogaster; Oxidative Stress
• Online Access: http://ijt.arakmu.ac.ir/browse.php?a_id=305&sid=1&slc_lang=en

Background: In this study, the toxicity of the different xenobiotics was tested on the fruit fly Drosophila melanogaster model system. Methods: Fly larvae were raised on food supplemented with xenobiotics at different concentrations (sodium nitroprusside (0.1-1.5 mM), S-nitrosoglutathione (0.5-4 mM), and potassium ferrocyanide (1 mM)). Emergence of flies, food intake by larvae, and pupation height preference as well as aconitase activity (in 2-day old flies) were measured. Results: Food supplementation with xenobiotics caused a developmental delay in the flies and decreased pupation height. Biochemical analyses of oxidative stress markers and activities of antioxidants and their associated enzymes were carried out on 2-day-old flies emerged from control larvae and larvae fed on food supplemented with chemicals. Larval exposure to chemicals resulted in lower activities of aconitase in flies of both sexes and perturbation in activities of antioxidant enzymes. Conclusions: The results of this study showed that among a variety of parameters tested, aconitase activity, developmental endpoints, and pupation height may be used as reliable early indicators of toxicity caused by different chemicals.