LncRNA HOTTIP-Mediated HOXA11 Expression Promotes Cell Growth, Migration and Inhibits Cell Apoptosis in Breast Cancer

• Main Authors: Yanqin Sun, Chao Zeng, Siyuan Gan, Hongmei Li, Ying Cheng, Dongjie Chen, Rujia Li, Wei Zhu
• Format: Article
• Language: English
• Published: MDPI AG 2018-02-01
• Series: International Journal of Molecular Sciences
• Subjects: HOTTIP; breast cancer; growth; migration; apoptosis
• Online Access: http://www.mdpi.com/1422-0067/19/2/472

As the most common cause of cancer death in women, the pathogenesis of breast cancer still remains unclear. Here, we reported a long non-coding RNA (lncRNA), HOTTIP (HOXA transcript at the distal tip), that may play an important role in the pathogenesis of breast cancer. Using gain-and-loss-of experiments in vitro and in vivo, we observed the marked upregulation of HOTTIP/HOXA11 in the breast cancer cell line, MCF-7, and the downregulation of HOTTIP or HOXA11, which might inhibit cell proliferation and migration but promote cell apoptosis in breast cancer MCF-7 cells. In addition, by further rescue experiments with HOXA11 overexpression, we uncovered a novel potential regulatory mechanism between HOTTIP and one of its physical HOXA clusters, HOXA11. Hence, HOTTIP may mediate, at least partly, HOXA11 expression involved in cell growth, migration, and apoptosis of breast cancer MCF-7 cells.