Biochemical Characterization of a Carboxylesterase from the Archaeon Pyrobaculum sp. 1860 and a Rational Explanation of Its Substrate Specificity and Thermostability

Biochemical Characterization of a Carboxylesterase from the Archaeon Pyrobaculum sp. 1860 and a Rational Explanation of Its Substrate Specificity and Thermostability

In this work, genome mining was used to identify esterase/lipase genes in the archaeon Pyrobaculum sp. 1860. A gene was cloned and functionally expressed in Escherichia coli as His-tagged protein. The recombinant enzyme (rP186_1588) was verified by western blotting and peptide mass fingerprinting. B...

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Bibliographic Details
Main Authors: Hua Shao, Li Xu, Yunjun Yan
Format: Article
Language:English
Published: MDPI AG 2014-09-01
Series:International Journal of Molecular Sciences
Subjects:
carboxylesterase
thermostability
structure modeling
docking
molecular dynamics
Online Access:http://www.mdpi.com/1422-0067/15/9/16885

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