Genetic predictors of the effectiveness of the standard low-calorie diet in obese patients

• Main Authors: Tatyana Sentsova, Olga Chernyak, Ilya Vorozhko, Kamila Gapparova, Olga Grigoryan, Yulya Chekhonina, Alexandra Churycheva
• Format: Article
• Language: English
• Published: Endocrinology Research Centre 2016-06-01
• Series: Ожирение и метаболизм
• Subjects: ген; ожирение; диетотерапия; ApoE; LPL; полиморфизмы
• Online Access: https://endojournals.ru/index.php/omet/article/view/7881

Objectives: The aim of the study was to investigate the genetic predictors of standard low-calorie diet at different polymorphic variations ApoE and LPL genes in obese patients. Materials and methods: Our study involved 88 obese patients aged 19 to 67 years (27 men and 61 women, mean age 38.40 ± 2.82 years and the average BMI 41.71 ± 1.23). Study of lipid metabolism includes the determination of total cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein determined by spectrophotometry and immuno turbidimetry. Analysis of polymorphisms of LPL gene Ser447Ter (c.1595C>G), Cys112Arg (c.388T>C) and Arg158Cys (c.526C>T) of ApoE gene was performed by polymerase chain reaction. Results: The results show significant differences in the efficacy of diet, depending on the genotype. We found that obese patients have significantly higher carrier genotypes c.388 T/T + c.526 C/C (by 20% reduced total cholesterol, by 19% – triglycerides, by20 % – low density lipoprotein, by 16% – high density lipoprotein, and by 9% – glucose), c.388 T/T + c.526 C/T ApoE gene (by 24% reduced total cholesterol, by 20% – triglycerides, and by 2% increased level of high-density lipoprotein) and C/C LPL gene (by 18% reduced total cholesterol concentration, by 17% – triglycerides, by 16% – high density lipoprotein, by 18% – low density lipoprotein, and by 9% – glucose). Conclusions: Thus, ApoE and LPL genes alleles can be used as biomarkers of prognosis of efficiency of a standard low-calorie diet therapy in obese patients.