Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.)
ABSTRACT <br /> Callus induction occurred on stem segments of Nigella sativa L. seedlings grown on a modified Murashige and Skoog medium containing 10-6 M of 2, 4- dichlorophenoxy acetic acid (2, 4 – D). <br /> Glutamine synthetase (EC 6. 3 . 1. 2) was isolated and partially purified fro...
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College of Education for Pure Sciences
2009-03-01
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| Series: | مجلة التربية والعلم |
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| Online Access: | https://edusj.mosuljournals.com/article_57421_81db50f49564b4fbb06a3f68cb81e240.pdf |
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doaj-5519874e851e40b7abd67de61313086b2020-11-25T02:26:14ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302009-03-01221849510.33899/edusj.2009.5742157421Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.)Nehal Ezzat0Sajeda Abood1Department of Biology, College of Science, University of Mosul, Mosul, IraqDepartment of Biology, College of Science, University of Mosul, Mosul, IraqABSTRACT <br /> Callus induction occurred on stem segments of Nigella sativa L. seedlings grown on a modified Murashige and Skoog medium containing 10-6 M of 2, 4- dichlorophenoxy acetic acid (2, 4 – D). <br /> Glutamine synthetase (EC 6. 3 . 1. 2) was isolated and partially purified from Nigella sativa L. callus the partially purified enzyme from the aqueous extract by ammonium sulfate precipitation, dialysis and gel filtration on sephadex G – 200 revealed that the activity increased by 37 fold. The molecular weight by gel – filtration chromatography was found to be around 50000 Da. The optimum conditions of the partially purified glutamine synthetase from callus were obtained by using Tris – HCl (80 mM) at pH 2.7 as a buffer and 10 mM of glutamate as substrate with incubation temperature 30° C. The Michaelis Menten constants for glutamate, adenosinetriphophate and ammonium chloride were 10 × 10-3M, 2 × 10-3 M and 0.280 × 10-3 M respectively.https://edusj.mosuljournals.com/article_57421_81db50f49564b4fbb06a3f68cb81e240.pdfisolationclotamine synthetaseblack bean plantnigella sativa l |
| collection |
DOAJ |
| language |
Arabic |
| format |
Article |
| sources |
DOAJ |
| author |
Nehal Ezzat Sajeda Abood |
| spellingShingle |
Nehal Ezzat Sajeda Abood Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.) مجلة التربية والعلم isolation clotamine synthetase black bean plant nigella sativa l |
| author_facet |
Nehal Ezzat Sajeda Abood |
| author_sort |
Nehal Ezzat |
| title |
Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.) |
| title_short |
Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.) |
| title_full |
Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.) |
| title_fullStr |
Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.) |
| title_full_unstemmed |
Isolation and partial purification of the clotamine synthetase from the black bean plant (Nigella sativa L.) |
| title_sort |
isolation and partial purification of the clotamine synthetase from the black bean plant (nigella sativa l.) |
| publisher |
College of Education for Pure Sciences |
| series |
مجلة التربية والعلم |
| issn |
1812-125X 2664-2530 |
| publishDate |
2009-03-01 |
| description |
ABSTRACT <br /> Callus induction occurred on stem segments of Nigella sativa L. seedlings grown on a modified Murashige and Skoog medium containing 10-6 M of 2, 4- dichlorophenoxy acetic acid (2, 4 – D). <br /> Glutamine synthetase (EC 6. 3 . 1. 2) was isolated and partially purified from Nigella sativa L. callus the partially purified enzyme from the aqueous extract by ammonium sulfate precipitation, dialysis and gel filtration on sephadex G – 200 revealed that the activity increased by 37 fold. The molecular weight by gel – filtration chromatography was found to be around 50000 Da. The optimum conditions of the partially purified glutamine synthetase from callus were obtained by using Tris – HCl (80 mM) at pH 2.7 as a buffer and 10 mM of glutamate as substrate with incubation temperature 30° C. The Michaelis Menten constants for glutamate, adenosinetriphophate and ammonium chloride were 10 × 10-3M, 2 × 10-3 M and 0.280 × 10-3 M respectively. |
| topic |
isolation clotamine synthetase black bean plant nigella sativa l |
| url |
https://edusj.mosuljournals.com/article_57421_81db50f49564b4fbb06a3f68cb81e240.pdf |
| work_keys_str_mv |
AT nehalezzat isolationandpartialpurificationoftheclotaminesynthetasefromtheblackbeanplantnigellasatival AT sajedaabood isolationandpartialpurificationoftheclotaminesynthetasefromtheblackbeanplantnigellasatival |
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