Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque
Aim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with IC...
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doaj-55174d5171464315ad8e422f9810dbee2021-05-31T23:08:05ZengMDPI AGDentistry Journal2304-67672021-05-019525210.3390/dj9050052Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental PlaqueSakari Nikinmaa0Niina Moilanen1Timo Sorsa2Juha Rantala3Heikki Alapulli4Anja Kotiranta5Petri Auvinen6Esko Kankuri7Jukka H. Meurman8Tommi Pätilä9Department of Neuroscience and Biomedical Engineering, Aalto University, 12200 Espoo, FinlandDepartment of Oral and Maxillofacial Diseases, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Neuroscience and Biomedical Engineering, Aalto University, 12200 Espoo, FinlandDepartment of Oral and Maxillofacial Diseases, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandInstitute of Biotechnology, University of Helsinki, 00790 Helsinki, FinlandFaculty of Medicine, Department of Pharmacology, University of Helsinki, 00290 Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, Faculty of Medicine, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, FinlandDepartment of Congenital Heart Surgery and Organ Transplantation, New Children’s Hospital, University of Helsinki, 00290 Helsinki, FinlandAim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100 J/cm<sup>2</sup> of 810 nm LED light was applied to the aPDT-treatment area. Plaque area and gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. Results: aPDT administration was preformed successfully and plaque-specifically with the combination of ICG and the applicator. Total plaque area and endpoint MMP-8 levels were reduced on the aPDT-treatment side. aPDT reduced <i>Streptococcus</i>, <i>Acinetobacteria</i>, <i>Capnocytophaga</i>, and <i>Rothia</i> bacteria species in plaques. Conclusion: ICG-assisted aPDT reduces plaque forming bacteria and exerts anti-inflammatory and anti-proteolytic effects.https://www.mdpi.com/2304-6767/9/5/52antibacterial photodynamic therapydental plaquegingivitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sakari Nikinmaa Niina Moilanen Timo Sorsa Juha Rantala Heikki Alapulli Anja Kotiranta Petri Auvinen Esko Kankuri Jukka H. Meurman Tommi Pätilä |
spellingShingle |
Sakari Nikinmaa Niina Moilanen Timo Sorsa Juha Rantala Heikki Alapulli Anja Kotiranta Petri Auvinen Esko Kankuri Jukka H. Meurman Tommi Pätilä Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque Dentistry Journal antibacterial photodynamic therapy dental plaque gingivitis |
author_facet |
Sakari Nikinmaa Niina Moilanen Timo Sorsa Juha Rantala Heikki Alapulli Anja Kotiranta Petri Auvinen Esko Kankuri Jukka H. Meurman Tommi Pätilä |
author_sort |
Sakari Nikinmaa |
title |
Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque |
title_short |
Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque |
title_full |
Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque |
title_fullStr |
Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque |
title_full_unstemmed |
Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque |
title_sort |
indocyanine green-assisted and led-light-activated antibacterial photodynamic therapy reduces dental plaque |
publisher |
MDPI AG |
series |
Dentistry Journal |
issn |
2304-6767 |
publishDate |
2021-05-01 |
description |
Aim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100 J/cm<sup>2</sup> of 810 nm LED light was applied to the aPDT-treatment area. Plaque area and gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. Results: aPDT administration was preformed successfully and plaque-specifically with the combination of ICG and the applicator. Total plaque area and endpoint MMP-8 levels were reduced on the aPDT-treatment side. aPDT reduced <i>Streptococcus</i>, <i>Acinetobacteria</i>, <i>Capnocytophaga</i>, and <i>Rothia</i> bacteria species in plaques. Conclusion: ICG-assisted aPDT reduces plaque forming bacteria and exerts anti-inflammatory and anti-proteolytic effects. |
topic |
antibacterial photodynamic therapy dental plaque gingivitis |
url |
https://www.mdpi.com/2304-6767/9/5/52 |
work_keys_str_mv |
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