Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion

Background. The detection of neoplastic transformation and prediction of the thera­peutic response are very important for effective cancer therapy. Current assessment of tumor treatment efficacy relies on evaluating changes in the tumor size or volume, weeks to months after the assumption of a thera...

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Main Authors: A. Babsky, N. Bansal
Format: Article
Language:English
Published: Львівський національний університет імені Івана Франка 2020-09-01
Series:Біологічні студії
Subjects:
Online Access:http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1142
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spelling doaj-54fd2a978a654d6e85384f7cf5ed52f52021-08-02T10:32:45ZengЛьвівський національний університет імені Івана ФранкаБіологічні студії1996-45362311-07832020-09-0114331210.30970/sbi.1403.625Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusionA. Babsky0https://orcid.org/0000-0001-5559-0456N. Bansal1Ivan Franko National University of Lviv, UkraineDepartment of Radiology and Imaging Sciences, Indiana University, Indianapolis, USABackground. The detection of neoplastic transformation and prediction of the thera­peutic response are very important for effective cancer therapy. Current assessment of tumor treatment efficacy relies on evaluating changes in the tumor size or volume, weeks to months after the assumption of a therapeutic protocol. The tissue perfusion is one of the most important parameter to estimate the neoplastic progress and the efficacy of antitumor therapy. 1H-magnetic resonance imaging (MRI) is an effective tool that provides distinctive information related to structural, cellular, apoptotic, and necrotic changes in tumor tissue. The technique can be used widely for tumor detection and monitoring of the response to treatment. Methods. Dynamic contrast enhanced 1H-MRI was used for the assessment of tumor perfusion parameters in normal muscle and in subcutaneous Radiation Infused Fibrosarcoma – 1 (RIF-1) developed under the skin in C3H mice. Gadolinium (20 mM) was used as a capillaries perfusion tracer. Therapy of RIF-1 was administered by a single intraperitoneal injection of 5-fluorouracil (150 mg/kg). MRI experiments were performed before and 3 days after the treatment. Results. Dynamic contrast enhanced 1H-MRI has shown a much lower perfusion rate in RIF-1 tumor compared to skeletal muscle. 5-fluorouracil caused a significant decrease in subcutaneous RIF-1 volume on days 2 and 3 post-treatment, as well as an increase in tumor inflow measured by Dynamic contrast enhanced 1H-MRI. An increase in tumor tissue perfusion correlated with an increase in tissue apparent diffusion coefficient and total Na+ concentration following 5-fluorouracil chemotherapy reflect an increase in extracellular space and vasodilatation. On the other hand, as it was shown in our previous publications, the lower intracellular Na+ concentration and glucose uptake in treated by 5-fluorouracil tumors compared with control tumors suggest a shift in tumor metabolism from glycolysis to oxidation and/or a decrease in cell density. Conclusions. Method of dynamic contrast enhanced 1H-MRI in combination with other NMR methods, positron emission tomography, histology, etc. should prove useful in assessment of neoplastic transformation of tumor capillaries and efficacy of chemotherapy. http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1142muscleperfusiongadoliniumfibrosarcoma5-fluorouracil1h-mri
collection DOAJ
language English
format Article
sources DOAJ
author A. Babsky
N. Bansal
spellingShingle A. Babsky
N. Bansal
Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
Біологічні студії
muscle
perfusion
gadolinium
fibrosarcoma
5-fluorouracil
1h-mri
author_facet A. Babsky
N. Bansal
author_sort A. Babsky
title Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
title_short Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
title_full Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
title_fullStr Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
title_full_unstemmed Dynamic contrast enhanced 1H-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
title_sort dynamic contrast enhanced 1h-magnetic resonance imaging in assessment of skeletal muscle and fibrosarcoma perfusion
publisher Львівський національний університет імені Івана Франка
series Біологічні студії
issn 1996-4536
2311-0783
publishDate 2020-09-01
description Background. The detection of neoplastic transformation and prediction of the thera­peutic response are very important for effective cancer therapy. Current assessment of tumor treatment efficacy relies on evaluating changes in the tumor size or volume, weeks to months after the assumption of a therapeutic protocol. The tissue perfusion is one of the most important parameter to estimate the neoplastic progress and the efficacy of antitumor therapy. 1H-magnetic resonance imaging (MRI) is an effective tool that provides distinctive information related to structural, cellular, apoptotic, and necrotic changes in tumor tissue. The technique can be used widely for tumor detection and monitoring of the response to treatment. Methods. Dynamic contrast enhanced 1H-MRI was used for the assessment of tumor perfusion parameters in normal muscle and in subcutaneous Radiation Infused Fibrosarcoma – 1 (RIF-1) developed under the skin in C3H mice. Gadolinium (20 mM) was used as a capillaries perfusion tracer. Therapy of RIF-1 was administered by a single intraperitoneal injection of 5-fluorouracil (150 mg/kg). MRI experiments were performed before and 3 days after the treatment. Results. Dynamic contrast enhanced 1H-MRI has shown a much lower perfusion rate in RIF-1 tumor compared to skeletal muscle. 5-fluorouracil caused a significant decrease in subcutaneous RIF-1 volume on days 2 and 3 post-treatment, as well as an increase in tumor inflow measured by Dynamic contrast enhanced 1H-MRI. An increase in tumor tissue perfusion correlated with an increase in tissue apparent diffusion coefficient and total Na+ concentration following 5-fluorouracil chemotherapy reflect an increase in extracellular space and vasodilatation. On the other hand, as it was shown in our previous publications, the lower intracellular Na+ concentration and glucose uptake in treated by 5-fluorouracil tumors compared with control tumors suggest a shift in tumor metabolism from glycolysis to oxidation and/or a decrease in cell density. Conclusions. Method of dynamic contrast enhanced 1H-MRI in combination with other NMR methods, positron emission tomography, histology, etc. should prove useful in assessment of neoplastic transformation of tumor capillaries and efficacy of chemotherapy.
topic muscle
perfusion
gadolinium
fibrosarcoma
5-fluorouracil
1h-mri
url http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1142
work_keys_str_mv AT ababsky dynamiccontrastenhanced1hmagneticresonanceimaginginassessmentofskeletalmuscleandfibrosarcomaperfusion
AT nbansal dynamiccontrastenhanced1hmagneticresonanceimaginginassessmentofskeletalmuscleandfibrosarcomaperfusion
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