PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3

• Main Authors: Zhifei Li, Dong Guo, Yali Qin, Mingzhou Chen
• Format: Article
• Language: English
• Published: Elsevier 2019-11-01
• Series: Cell Reports
• Online Access: http://www.sciencedirect.com/science/article/pii/S221112471931366X

Summary: Many positive-strand RNA viruses remodel the endomembrane to form specialized replication organelles. However, knowledge regarding whether negative-strand RNA viruses take advantage of intracellular membranes for replication is limited. Here we show that a negative-strand RNA virus, human parainfluenza virus type 3 (HPIV3), remodels the endoplasmic reticulum (ER) membrane to form inclusion bodies (IBs), whereby the phosphoprotein (P) of HPIV3 recruits phosphatidylinositol 4-kinase beta (PI4KB) to IBs to generate PI4P, creating a PI4P-enriched microenvironment to promote HPIV3 replication. In addition, we find that human respiratory syncytial virus (HRSV) also takes advantage of the ER to form IBs and that these IBs are also enriched with PI4P. The nucleoprotein of HRSV recruits PI4KB to IBs. These results suggest that paramyxoviruses also exploit the host endomembrane to form IBs and that PI4KB is recruited by viral proteins to enrich IBs with PI4P to facilitate viral replication. : Many positive-strand RNA viruses reorganize the endomembrane to form specialized replication organelles. However, whether negative-strand RNA viruses use intracellular membranes for replication is largely unknown. Li et al. demonstrate that inclusion bodies of HPIV3 induce ER membrane rearrangement. PI4KB is recruited to IBs to generate PI4P, which facilitates viral replication. Keywords: PI4KB, IBs, ER membrane, HPIV3