Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins

The low throughput or content of current methods for the analysis of glycans-glycan binding proteins (GBPs) interactions hampers their clinical applications. Here, the authors conjugate synthesized glycans to Luminex beads to detect GBPs and apply it for the discovery of ovarian cancer biomarkers.

Bibliographic Details
Main Authors:	Sharad Purohit, Tiehai Li, Wanyi Guan, Xuezheng Song, Jing Song, Yanna Tian, Lei Li, Ashok Sharma, Boying Dun, David Mysona, Sharad Ghamande, Bunja Rungruang, Richard D. Cummings, Peng George Wang, Jin-Xiong She
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-017-02747-y

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spelling	doaj-54bd91a6bdc84bbaaee3bb8a242ecc342021-05-11T10:32:19ZengNature Publishing GroupNature Communications2041-17232018-01-019111210.1038/s41467-017-02747-yMultiplex glycan bead array for high throughput and high content analyses of glycan binding proteinsSharad Purohit0Tiehai Li1Wanyi Guan2Xuezheng Song3Jing Song4Yanna Tian5Lei Li6Ashok Sharma7Boying Dun8David Mysona9Sharad Ghamande10Bunja Rungruang11Richard D. Cummings12Peng George Wang13Jin-Xiong She14Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta UniversityDepartment of Chemistry, Georgia State UniversityDepartment of Chemistry, Georgia State UniversityDepartment of Biochemistry, Emory University School of MedicineDepartment of Chemistry, Georgia State UniversityDepartment of Obstetrics and Gynecology, Medical College of Georgia, Augusta UniversityDepartment of Chemistry, Georgia State UniversityCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta UniversityCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta UniversityCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta UniversityDepartment of Obstetrics and Gynecology, Medical College of Georgia, Augusta UniversityDepartment of Obstetrics and Gynecology, Medical College of Georgia, Augusta UniversityDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical SchoolDepartment of Chemistry, Georgia State UniversityCenter for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta UniversityThe low throughput or content of current methods for the analysis of glycans-glycan binding proteins (GBPs) interactions hampers their clinical applications. Here, the authors conjugate synthesized glycans to Luminex beads to detect GBPs and apply it for the discovery of ovarian cancer biomarkers.https://doi.org/10.1038/s41467-017-02747-y
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sharad Purohit Tiehai Li Wanyi Guan Xuezheng Song Jing Song Yanna Tian Lei Li Ashok Sharma Boying Dun David Mysona Sharad Ghamande Bunja Rungruang Richard D. Cummings Peng George Wang Jin-Xiong She
spellingShingle	Sharad Purohit Tiehai Li Wanyi Guan Xuezheng Song Jing Song Yanna Tian Lei Li Ashok Sharma Boying Dun David Mysona Sharad Ghamande Bunja Rungruang Richard D. Cummings Peng George Wang Jin-Xiong She Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins Nature Communications
author_facet	Sharad Purohit Tiehai Li Wanyi Guan Xuezheng Song Jing Song Yanna Tian Lei Li Ashok Sharma Boying Dun David Mysona Sharad Ghamande Bunja Rungruang Richard D. Cummings Peng George Wang Jin-Xiong She
author_sort	Sharad Purohit
title	Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
title_short	Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
title_full	Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
title_fullStr	Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
title_full_unstemmed	Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
title_sort	multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-01-01
description	The low throughput or content of current methods for the analysis of glycans-glycan binding proteins (GBPs) interactions hampers their clinical applications. Here, the authors conjugate synthesized glycans to Luminex beads to detect GBPs and apply it for the discovery of ovarian cancer biomarkers.
url	https://doi.org/10.1038/s41467-017-02747-y
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Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins

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