Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

Abstract Background There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA. Therefore, depletion of globin gene RNA an...

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Bibliographic Details
Main Authors: Jin Sung Jang, Brianna Berg, Eileen Holicky, Bruce Eckloff, Mark Mutawe, Minerva M. Carrasquillo, Nilüfer Ertekin-Taner, Julie M. Cuninngham
Format: Article
Language:English
Published: BMC 2020-12-01
Series:BMC Genomics
Subjects:
mRNA-Seq
Globin gene depletion
rRNA
Whole blood
Online Access:https://doi.org/10.1186/s12864-020-07304-4

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