Fractionation of fecal neutral steroids by high performance liquid chromatography.

Fractionation of fecal neutral steroids by high performance liquid chromatography.

Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5-3 beta-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 9...

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Bibliographic Details
Main Authors: E M Jackson, C A Kloss, S T Weintraub, G E Mott
Format: Article
Language:English
Published: Elsevier 1985-07-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520343212
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spelling doaj-53f71da213b94083a8a928d354f8c39c2021-04-25T04:15:20ZengElsevierJournal of Lipid Research0022-22751985-07-01267893897Fractionation of fecal neutral steroids by high performance liquid chromatography.E M JacksonC A KlossS T WeintraubG E MottFecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5-3 beta-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described.http://www.sciencedirect.com/science/article/pii/S0022227520343212
collection DOAJ
language English
format Article
sources DOAJ
author E M Jackson
C A Kloss
S T Weintraub
G E Mott
spellingShingle E M Jackson
C A Kloss
S T Weintraub
G E Mott
Fractionation of fecal neutral steroids by high performance liquid chromatography.
Journal of Lipid Research
author_facet E M Jackson
C A Kloss
S T Weintraub
G E Mott
author_sort E M Jackson
title Fractionation of fecal neutral steroids by high performance liquid chromatography.
title_short Fractionation of fecal neutral steroids by high performance liquid chromatography.
title_full Fractionation of fecal neutral steroids by high performance liquid chromatography.
title_fullStr Fractionation of fecal neutral steroids by high performance liquid chromatography.
title_full_unstemmed Fractionation of fecal neutral steroids by high performance liquid chromatography.
title_sort fractionation of fecal neutral steroids by high performance liquid chromatography.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1985-07-01
description Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5-3 beta-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described.
url http://www.sciencedirect.com/science/article/pii/S0022227520343212
work_keys_str_mv AT emjackson fractionationoffecalneutralsteroidsbyhighperformanceliquidchromatography
AT cakloss fractionationoffecalneutralsteroidsbyhighperformanceliquidchromatography
AT stweintraub fractionationoffecalneutralsteroidsbyhighperformanceliquidchromatography
AT gemott fractionationoffecalneutralsteroidsbyhighperformanceliquidchromatography
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