Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes

In human peripheral blood lymphocytes (PBL) monosialoganglioside GM3 appears to be the major ganglioside on the cell plasma membrane. We have analyzed the expression and distribution pattern of GM3 molecules on the lymphocyte plasma membrane by flow cytometry, immunofluorescence, and immunoelectron...

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Main Authors: M Sorice, I Parolini, T Sansolini, T Garofalo, V Dolo, M Sargiacomo, T Tai, C Peschle, M R Torrisi, A Pavan
Format: Article
Language:English
Published: Elsevier 1997-05-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520372217
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spelling doaj-53dd9559a04d413eb80f523bb71928122021-04-26T05:47:58ZengElsevierJournal of Lipid Research0022-22751997-05-01385969980Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytesM Sorice0I Parolini1T Sansolini2T Garofalo3V Dolo4M Sargiacomo5T Tai6C Peschle7M R Torrisi8A Pavan9Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Italy.In human peripheral blood lymphocytes (PBL) monosialoganglioside GM3 appears to be the major ganglioside on the cell plasma membrane. We have analyzed the expression and distribution pattern of GM3 molecules on the lymphocyte plasma membrane by flow cytometry, immunofluorescence, and immunoelectron microscopy, using an anti-GM3 monoclonal antibody. Both CD4+ and CD8+ T lymphocyte subpopulations showed substantial GM3 expression, as determined by thin-layer chromatography and flow cytometric analysis. A clustered distribution of GM3 molecules on the cell surface, revealed by immunofluorescence and immunogold electron microscopy, clearly indicated the presence of GM3 molecule-enriched plasma membrane domains. To better define these domains, we analyzed the ganglioside and protein composition of buoyant low-density Triton-insoluble (LDTI) lymphocyte fractions. The results show that GM3 is enriched approximately 20-fold in LDTI fraction, as compared with total cell lysates. In addition, CD4 and lck molecules are selectively recovered in the same LDTI fraction isolated from human PBL. These findings, together with the observation that anti-CD4 co-immunoprecipitated GM3, support the hypothesis of a possible GM3-CD4 interaction and suggest a role for gangliosides as structural components of the membrane multimolecular signaling complex involved in T-cell activation, antigen recognition, and other dynamic lymphocytic plasma membrane functions.http://www.sciencedirect.com/science/article/pii/S0022227520372217
collection DOAJ
language English
format Article
sources DOAJ
author M Sorice
I Parolini
T Sansolini
T Garofalo
V Dolo
M Sargiacomo
T Tai
C Peschle
M R Torrisi
A Pavan
spellingShingle M Sorice
I Parolini
T Sansolini
T Garofalo
V Dolo
M Sargiacomo
T Tai
C Peschle
M R Torrisi
A Pavan
Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
Journal of Lipid Research
author_facet M Sorice
I Parolini
T Sansolini
T Garofalo
V Dolo
M Sargiacomo
T Tai
C Peschle
M R Torrisi
A Pavan
author_sort M Sorice
title Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
title_short Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
title_full Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
title_fullStr Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
title_full_unstemmed Evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
title_sort evidence for the existence of ganglioside-enriched plasma membrane domains in human peripheral lymphocytes
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1997-05-01
description In human peripheral blood lymphocytes (PBL) monosialoganglioside GM3 appears to be the major ganglioside on the cell plasma membrane. We have analyzed the expression and distribution pattern of GM3 molecules on the lymphocyte plasma membrane by flow cytometry, immunofluorescence, and immunoelectron microscopy, using an anti-GM3 monoclonal antibody. Both CD4+ and CD8+ T lymphocyte subpopulations showed substantial GM3 expression, as determined by thin-layer chromatography and flow cytometric analysis. A clustered distribution of GM3 molecules on the cell surface, revealed by immunofluorescence and immunogold electron microscopy, clearly indicated the presence of GM3 molecule-enriched plasma membrane domains. To better define these domains, we analyzed the ganglioside and protein composition of buoyant low-density Triton-insoluble (LDTI) lymphocyte fractions. The results show that GM3 is enriched approximately 20-fold in LDTI fraction, as compared with total cell lysates. In addition, CD4 and lck molecules are selectively recovered in the same LDTI fraction isolated from human PBL. These findings, together with the observation that anti-CD4 co-immunoprecipitated GM3, support the hypothesis of a possible GM3-CD4 interaction and suggest a role for gangliosides as structural components of the membrane multimolecular signaling complex involved in T-cell activation, antigen recognition, and other dynamic lymphocytic plasma membrane functions.
url http://www.sciencedirect.com/science/article/pii/S0022227520372217
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