Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding

Rieu et al. present a magnetic tweezers based single-molecule manipulation method, called kinetic locking, for direct detection of biomolecular binding without use of fluorescent probes. By measuring dynamical interactions of E. coli RecQ helicase with its DNA substrate, authors show that this metho...

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Bibliographic Details
Main Authors: Martin Rieu, Jessica Valle-Orero, Bertrand Ducos, Jean-François Allemand, Vincent Croquette
Format: Article
Language:English
Published: Nature Publishing Group 2021-09-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-02606-z