Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)

Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)

Andrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method.The eluation was carried by out gradiently using methanol: water as an eluent and U...

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Bibliographic Details
Main Author: Toetik Aryani
Format: Article
Language:English
Published: Indonesian Biological Society 2012-02-01
Series:Journal of Biological Researches
Subjects:
andrographis paniculata Nees
andrographolide
HPLC
Online Access:http://berkalahayati.org/journal/dc4876f3
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spelling doaj-53bd764155844e8aa6b0b11a0e29af282020-11-24T21:00:27ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2012-02-01111737610.23869/bphjbr.11.1.200512Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)Toetik Aryani0Bagian Ilmu Biomedik Fakultas Farmasi Universitas AirlanggaAndrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method.The eluation was carried by out gradiently using methanol: water as an eluent and UV spectrophotometer at maks 228 as detector. The result of HPLC analysis are selectivity >1.2–1.5; r was 0.9937; precision was KV < 10%; accuracy > 90%; DL was 0.075; QL was 0.50 and PW < F table. Andrographolide content of Andrographis paniculata Nees from Banyuwangi was 6.25%, Kediri was 14.69% and Surabaya was 6.83% http://berkalahayati.org/journal/dc4876f3andrographis paniculata NeesandrographolideHPLC
collection DOAJ
language English
format Article
sources DOAJ
author Toetik Aryani
spellingShingle Toetik Aryani
Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)
Journal of Biological Researches
andrographis paniculata Nees
andrographolide
HPLC
author_facet Toetik Aryani
author_sort Toetik Aryani
title Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)
title_short Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)
title_full Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)
title_fullStr Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)
title_full_unstemmed Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis panuculata Ness)
title_sort pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (kckt) dengan eluasi gradien terhadap ekstrak herba sambiloto (andrographis panuculata ness)
publisher Indonesian Biological Society
series Journal of Biological Researches
issn 0852-6834
2337-389X
publishDate 2012-02-01
description Andrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method.The eluation was carried by out gradiently using methanol: water as an eluent and UV spectrophotometer at maks 228 as detector. The result of HPLC analysis are selectivity >1.2–1.5; r was 0.9937; precision was KV < 10%; accuracy > 90%; DL was 0.075; QL was 0.50 and PW < F table. Andrographolide content of Andrographis paniculata Nees from Banyuwangi was 6.25%, Kediri was 14.69% and Surabaya was 6.83%
topic andrographis paniculata Nees
andrographolide
HPLC
url http://berkalahayati.org/journal/dc4876f3
work_keys_str_mv AT toetikaryani pengujianvalidasianalisiskadarandrografolidsecarakromatograficairkinerjatinggikcktdenganeluasigradienterhadapekstrakherbasambilotoandrographispanuculataness
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