Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of diffe...
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doaj-53a6465950a9416c95261dbe27ee79be2020-11-25T01:47:09ZengMDPI AGNanomaterials2079-49912020-01-0110224010.3390/nano10020240nano10020240Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not MatterLaurent Marichal0Géraldine Klein1Jean Armengaud2Yves Boulard3Stéphane Chédin4Jean Labarre5Serge Pin6Jean-Philippe Renault7Jean-Christophe Aude8Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceLaboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, 30207 Bagnols-sur-Cèze, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceLaboratoire Interdisciplinaire sur l’Organisation Nanométrique et Supramoléculaire, CEA, CNRS, NIMBE, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceLaboratoire Interdisciplinaire sur l’Organisation Nanométrique et Supramoléculaire, CEA, CNRS, NIMBE, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceBiomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media.https://www.mdpi.com/2079-4991/10/2/240silica nanoparticlesprotein coronacurvature effecthigh-throughput proteomicsbayesian statistical analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laurent Marichal Géraldine Klein Jean Armengaud Yves Boulard Stéphane Chédin Jean Labarre Serge Pin Jean-Philippe Renault Jean-Christophe Aude |
spellingShingle |
Laurent Marichal Géraldine Klein Jean Armengaud Yves Boulard Stéphane Chédin Jean Labarre Serge Pin Jean-Philippe Renault Jean-Christophe Aude Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter Nanomaterials silica nanoparticles protein corona curvature effect high-throughput proteomics bayesian statistical analysis |
author_facet |
Laurent Marichal Géraldine Klein Jean Armengaud Yves Boulard Stéphane Chédin Jean Labarre Serge Pin Jean-Philippe Renault Jean-Christophe Aude |
author_sort |
Laurent Marichal |
title |
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter |
title_short |
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter |
title_full |
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter |
title_fullStr |
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter |
title_full_unstemmed |
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter |
title_sort |
protein corona composition of silica nanoparticles in complex media: nanoparticle size does not matter |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2020-01-01 |
description |
Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media. |
topic |
silica nanoparticles protein corona curvature effect high-throughput proteomics bayesian statistical analysis |
url |
https://www.mdpi.com/2079-4991/10/2/240 |
work_keys_str_mv |
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