Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter

Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of diffe...

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Main Authors: Laurent Marichal, Géraldine Klein, Jean Armengaud, Yves Boulard, Stéphane Chédin, Jean Labarre, Serge Pin, Jean-Philippe Renault, Jean-Christophe Aude
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Nanomaterials
Subjects:
Online Access:https://www.mdpi.com/2079-4991/10/2/240
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spelling doaj-53a6465950a9416c95261dbe27ee79be2020-11-25T01:47:09ZengMDPI AGNanomaterials2079-49912020-01-0110224010.3390/nano10020240nano10020240Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not MatterLaurent Marichal0Géraldine Klein1Jean Armengaud2Yves Boulard3Stéphane Chédin4Jean Labarre5Serge Pin6Jean-Philippe Renault7Jean-Christophe Aude8Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceLaboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, 30207 Bagnols-sur-Cèze, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceLaboratoire Interdisciplinaire sur l’Organisation Nanométrique et Supramoléculaire, CEA, CNRS, NIMBE, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceLaboratoire Interdisciplinaire sur l’Organisation Nanométrique et Supramoléculaire, CEA, CNRS, NIMBE, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceBiomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media.https://www.mdpi.com/2079-4991/10/2/240silica nanoparticlesprotein coronacurvature effecthigh-throughput proteomicsbayesian statistical analysis
collection DOAJ
language English
format Article
sources DOAJ
author Laurent Marichal
Géraldine Klein
Jean Armengaud
Yves Boulard
Stéphane Chédin
Jean Labarre
Serge Pin
Jean-Philippe Renault
Jean-Christophe Aude
spellingShingle Laurent Marichal
Géraldine Klein
Jean Armengaud
Yves Boulard
Stéphane Chédin
Jean Labarre
Serge Pin
Jean-Philippe Renault
Jean-Christophe Aude
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
Nanomaterials
silica nanoparticles
protein corona
curvature effect
high-throughput proteomics
bayesian statistical analysis
author_facet Laurent Marichal
Géraldine Klein
Jean Armengaud
Yves Boulard
Stéphane Chédin
Jean Labarre
Serge Pin
Jean-Philippe Renault
Jean-Christophe Aude
author_sort Laurent Marichal
title Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
title_short Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
title_full Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
title_fullStr Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
title_full_unstemmed Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
title_sort protein corona composition of silica nanoparticles in complex media: nanoparticle size does not matter
publisher MDPI AG
series Nanomaterials
issn 2079-4991
publishDate 2020-01-01
description Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media.
topic silica nanoparticles
protein corona
curvature effect
high-throughput proteomics
bayesian statistical analysis
url https://www.mdpi.com/2079-4991/10/2/240
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