Regeneration of whole plants of geranium from petioles cultured in vitro

• Main Authors: Barbara Stefaniak, Maciej Zenkteler
• Format: Article
• Language: English
• Published: Polish Botanical Society 2014-01-01
• Series: Acta Societatis Botanicorum Poloniae
• Online Access: https://pbsociety.org.pl/journals/index.php/asbp/article/view/3238

Studies were made on the morphogenetic potential of petioles of Pelargonium hortorum (1 variety, 2 clones) and Pelargonium peltatum (1 variety, 1 clone) grown on the medium of Murashige and Skoog (MS) supplemented with auxins (IAA, IBA, NAA) and cytokinins (KIN, BAP, zeatin). The most intensive growth of callus of both species was observed on medium supplemented with 0.1 mg/l NAA and 10 mg/l KIN, both under constant illumination and in darkness. Differentiation of callus and regeneration of plants occurred in P. peltatum variety "PAC Dresdner Amethyst" and in P. hortorum clone 3766/4 on medium containing 1 mg/l BAP, 1 mg/l IAA and exposed to a photoperiod (L:D = 16:8).