| Summary: | <p>Abstract</p> <p>Background</p> <p>Simulation of DNA-microarray data serves at least three purposes: (i) optimizing the design of an intended DNA microarray experiment, (ii) comparing existing pre-processing and processing methods for best analysis of a given DNA microarray experiment, (iii) educating students, lab-workers and other researchers by making them aware of the many factors influencing DNA microarray experiments.</p> <p>Results</p> <p>Our model has multiple layers of factors influencing the experiment. The relative influence of such factors can differ significantly between labs, experiments within labs, etc. Therefore, we have added a module to roughly estimate their parameters from a given data set. This guarantees that our simulated data mimics real data as closely as possible.</p> <p>Conclusion</p> <p>We introduce a model for the simulation of dual-dye cDNA-microarray data closely resembling real data and coin the model and its software implementation "<it>SIMAGE</it>" which stands for simulation of microarray gene expression data. The software is freely accessible at: <url>http://bioinformatics.biol.rug.nl/websoftware/simage</url>.</p>
|
|---|
