Herbal Gel Formulation Developed for Anti-Human Immunodeficiency Virus (HIV)-1 Activity Also Inhibits In Vitro HSV-2 Infection

• Main Authors: Nripendra Nath Mishra, Ajay Kesharwani, Aakanksha Agarwal, Suja Kizhiyedath Polachira, Reshmi Nair, Satish Kumar Gupta
• Format: Article
• Language: English
• Published: MDPI AG 2018-10-01
• Series: Viruses
• Subjects: anti-HSV-2 activity; anti-HIV-1 activity; herbal gel formulation; viability of lactobacilli; pro-inflammatory cytokines
• Online Access: https://www.mdpi.com/1999-4915/10/11/580

Herpes simplex virus-2 (HSV-2) infection is the most common cause of genital ulcers. The impact of ulcers also demonstrates a strong link to the human immunodeficiency virus (HIV) infection. Complications, drug resistance, and side-effects of anti-viral drugs make the treatment of HSV-2 infection challenging. Herbal medicines have shown potential against HSV-2 and HIV infections. In this context, polyherbal gel formulation comprising 50% ethanolic extracts from <i>Acacia catechu</i>, <i>Lagerstroemia speciosa</i>, <i>Terminalia chebula</i> and <i>Phyllanthus emblica</i> has been developed. The gel formulation significantly exhibited virucidal activity against both HIV-1 and HSV-2 infections with IC₅₀, 55.93 &#177; 5.30 &#181;g/mL and 27.26 &#177; 4.87 &#181;g/mL, respectively. It also inhibited HSV-2 attachment and penetration to the Vero cells with an IC₅₀ = 46.55 &#177; 1.25 &#181;g/mL and 54.94 &#177; 2.52 &#181;g/mL respectively, which were significantly lower than acyclovir. However, acyclovir is more potent in post-infection assay with an IC₅₀ = 0.065 &#177; 0.01 &#181;g/mL whereas gel formulation showed an IC₅₀ = 469.05 &#177; 16.65 &#181;g/mL under similar conditions. Gel formulation showed no inhibitory effect on the viability of lactobacilli, human vaginal keratinocyte cells (Vk2/E6E7), and the integrity of the Caco-2 cells monolayer. Gel formulation did not lead to any significant increase in the secretion of pro-inflammatory cytokines and mutagenic index. The proposed gel formulation may be a promising candidate microbicide for the prevention of sexually transmitted HIV-1 and HSV-2.