Evaluation of the effectiveness of potato improvement in in vitro culture on optimized nutrient media

• Main Authors: Gneusheva I.A., Gavrilova A.Yu., Gagarina I.N., Gorkova I.V., Solokhina I.Yu.
• Format: Article
• Language: English
• Published: EDP Sciences 2021-01-01
• Series: BIO Web of Conferences
• Online Access: https://www.bio-conferences.org/articles/bioconf/pdf/2021/08/bioconf_fsraaba2021_05007.pdf

Studies have been conducted on the use of an optimized Murashige-Scoog nutrient medium to obtain healthy seeds of promising potato varieties. It was found that the use of agarized modified Murashige-Scoog nutrient medium with the addition of exometabolites of fungi of the genus Trichoderma lixii 1.5 mg/l with antimicrobial properties and the antiviral drug “Cycloferon” in a concentration of 0.01% contributes to an increase in the number of virus-free potato regenerants when the method of apical meristems and in vitro improvement is used together. The effectiveness of using the modified medium is confirmed by the following criteria: active growth of test tube potato micro-plants, which is expressed in optimal morphometric indicators on the example of the potato variety “Rosara”; the size of the isolated meristems is 10 mm, which contributes to the output of healthy plants; the survival rate of regenerating plants. It is shown that when using the modified medium, an average of 35-50% of regenerating plants were obtained, depending on the potato variety, free from M, S viruses.