Amorphous nanosilica induce endocytosis-dependent ROS generation and DNA damage in human keratinocytes

• Main Authors: Hirai Toshiro, Kondoh Sayuri, Tochigi Saeko, Nakazato Yasutaro, Matsuyama Keigo, Yoshikawa Tomoaki, Nabeshi Hiromi, Akase Takanori, Nagano Kazuya, Abe Yasuhiro, Yoshioka Yasuo, Kamada Haruhiko, Itoh Norio, Tsunoda Shin-ichi, Tsutsumi Yasuo
• Format: Article
• Language: English
• Published: BMC 2011-01-01
• Series: Particle and Fibre Toxicology
• Online Access: http://www.particleandfibretoxicology.com/content/8/1/1

<p>Abstract</p> <p>Background</p> <p>Clarifying the physicochemical properties of nanomaterials is crucial for hazard assessment and the safe application of these substances. With this in mind, we analyzed the relationship between particle size and the <it>in vitro </it>effect of amorphous nanosilica (nSP). Specifically, we evaluated the relationship between particle size of nSP and the <it>in vitro </it>biological effects using human keratinocyte cells (HaCaT).</p> <p>Results</p> <p>Our results indicate that exposure to nSP of 70 nm diameter (nSP70) induced an elevated level of reactive oxygen species (ROS), leading to DNA damage. A markedly reduced response was observed using submicron-sized silica particles of 300 and 1000 nm diameter. In addition, cytochalasin D-treatment reduced nSP70-mediated ROS generation and DNA damage, suggesting that endocytosis is involved in nSP70-mediated cellular effects.</p> <p>Conclusions</p> <p>Thus, particle size affects amorphous silica-induced ROS generation and DNA damage of HaCaT cells. We believe clarification of the endocytosis pathway of nSP will provide useful information for hazard assessment as well as the design of safer forms of nSPs.</p>