EXPRESSION OF SOME ANTIOXIDANT GENES IN SUNFLOWER INFECTED WITH BROOMRAPE

• Main Authors: Tatiana Sestecova, Ion Gisca, Aliona Cuccreavii, Olesea Tabara, Angela Port, Maria Duca
• Format: Article
• Language: English
• Published: "Alexandru Ioan Cuza" University of Iași 2015-09-01
• Series: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
• Online Access: http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/1158

Expression levels of ROS-scavenging genes (MnSODI, APX3 and AOX1A) in leaves (R5 stage; 90 days after sowing) of seven sunflower genotypes infected with three Orobanche cumana Wallr. populations were assayed in plants with/without broomrape aerial shoots and control group. Five lines were highly susceptible to all three populations. MS- 2161A was resistant and MS-2039A was tolerant to broomrape populations. The expression of studied genes was much more altered in highly susceptible genotypes than in those resistant. Significant differences in number of cases of ROS- scavenging genes with modified transcriptional activity in infected and non-symptomatic plants were not ascertained. The transcriptional activity of MnSODI, APX3 and AOX1A genes was weakly influenced by infection with broomrape (67 % cases) or was down-regulated (24 % cases). Some up-regulation cases (9 %) for MnSODI (MS-2039) and AOX1A gene (MS-2067) were revealed. AOX1A was the most responsive gene, especially when infection was produced by population from Anenii Noi.