Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.

Dermatopontin (DPT) is an extracellular matrix (ECM) protein with diversified pharmaceutical applications. It plays important role in cell adhesion/migration, angiogenesis and ECM maintenance. The recombinant production of this protein will enable further exploration of its multifaceted functions. I...

Full description

Bibliographic Details
Main Authors:	Trikkur Madom Seetaraman Amritha, Shubham Mahajan, Kumar Subramaniam, Yamini Chandramohan, Anuradha Dhanasekaran
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2020-01-01
Series:	PLoS ONE
Online Access:	https://doi.org/10.1371/journal.pone.0242798

Similar Items

Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli
by: L. Avilán, et al.
Published: (1997-12-01)

Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli
by: Sriram Padmanabhan, et al.
Published: (2011-03-01)

Expression and purification of recombinant hemoglobin in Escherichia coli.
by: Chandrasekhar Natarajan, et al.
Published: (2011-01-01)

Purification and refolding of recombinant viral protein expressed in Escherichia coli
by: Lai, Wen Bin
Published: (2003)

Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
by: Taeyeon Kim, et al.
Published: (2019-04-01)

Dermatopontin Modulates Cell Cycle Progression of Cancer cells
by: Ka-Hou Chon, et al.
Published: (2006)

Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase.
by: Yvonne D Trigoso, et al.
Published: (2016-01-01)

Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
by: Ram Shankar, et al.
Published: (2021-08-01)

Molecular cloning of a Clostridium B-xylosidase gene and enzyme purification from the recombinant Escherichia coli strain
by: Chambers, Kathleen Erin
Published: (1989)

Cloning, purification and kinetic studies of Escherichia coli RNase HII
by: Bostock, James A.
Published: (2007)

Gene Cloning and Expression of Recombinant Avian Interlukin 18 in Escherichia coli
by: Chun-Hsiang Huang, et al.
Published: (2015)

Cloning, optimization of periplasmic expression and purification of recombinant granulocyte macrophage-stimulating factor in Escherichia coli BL21 (DE3)
by: Elham Taherian, et al.
Published: (2019-01-01)

Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli
by: Meraj Pourhossein, et al.
Published: (2014-01-01)

Cloning, expression and purification of fructosyl amino acid oxidase (FAOX) in Escherichia Coli
by: Ho Ta Giap, et al.
Published: (2021-03-01)

Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System
by: Abbas Mohajeri, et al.
Published: (2016-06-01)

Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterization
by: Aliakbar Fazaeli, et al.
Published: (2018-11-01)

Purification and characterization of recombinant human p21 protein expressed in Escherichia coli
by: Kuo-Pao Lai, et al.
Published: (2000)

Recombinant expression of Verrucomicrobium spinosum tyrosinase in Escherichia coli, its purification and characterisation
by: Winroth, Lena
Published: (2019)

Cloning, Characterization and Application of Recombinant Carbonic Anhydrase in Escherichia coli
by: Tai-haoHe, et al.
Published: (2019)

Optimal cloning of PCR fragments by homologous recombination in Escherichia coli.
by: Ana Paula Jacobus, et al.
Published: (2015-01-01)

Cloning and expression of recombinant thrombin in Escherichia coli JM109 (DE3)
by: Vu, Thi Bich Ngoc, et al.
Published: (2017)

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli
by: Pollyana Pfrimer, et al.
Published: (2010-01-01)

High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG.
by: Dan Luo, et al.
Published: (2016-01-01)

Expression, purification and characterisation of recombinant catalase-peroxidases from Mycobacterium tuberculosis and Escherichia coli
by: Nagy, Judit
Published: (1997)

Expression, purification, and bioactivity of a soluble recombinant ovine interferon-tau in Escherichia coli
by: Yu Hai-Yang, et al.
Published: (2021-01-01)

Expression and purification of recombinant human trefoil factor 1 by Escherichia coli and Lactococcus lactis
by: Meng-Ting Lu, et al.
Published: (2011)

High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli
by: Loren E. Smith, et al.
Published: (2012-08-01)

Cloning and Expression of Human Keratinocyte Growth Factor in Escherichia coli for Recombinant Drug Production
by: Ebrahimzadeh, et al.
Published: (2014-09-01)

Molecular cloning, expression and characterization of the recombinant nattokinase from Bacillus subtilis in Escherichia coli.
by: Pe-Yin Huang, et al.
Published: (2007)

Cloning and Expression of Recombinant Human Matrix Metalloproteinase-1 from Escherichia coli Expression System
by: Yen Chou, et al.
Published: (2010)

Complete solubilization and purification of recombinant human growth hormone produced in Escherichia coli.
by: Min-Ji Kim, et al.
Published: (2013-01-01)

Rationale for the selection of aqueous two-phase systems for the purification of recombinant proteins expressed in Escherichia coli
by: Kaul, Anita
Published: (1995)

The 2'-5' RNA ligase of Escherichia coli : purification, cloning, and investigations of in vivo function
by: Arn, Eric
Published: (1996)

Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli
by: Christian Sonnendecker, et al.
Published: (2017-05-01)

High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in <it>Escherichia coli</it>
by: Huang Yadong, et al.
Published: (2010-02-01)

Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
by: Lisa Gerner, et al.
Published: (2016-09-01)

Purification of Soluble Recombinant Salmonella typhimurium Flagellin (FliC) Protein Constructs Expressed in Escherichia coli
by: Hooker, Jennifer Ann
Published: (2014)

Expression and purification of recombinant human proopiomelanocortin proteins in Escherichia coli : a combined biological and immunological approach
by: Grewal, Tarlochan Singh
Published: (1991)

Expression and purification of recombinant transactivation domain of hepatitis B virus (HBV) X protein in Escherichia coli
by: Han-Lin Jou, et al.
Published: (2010)

Cloning, expression, purification and identification of recombinant Staphylococcal classical enterotoxins.
by: HUANG,BAO-YUE, et al.
Published: (2016)