Antioxidant capacity of N’-méthylferrocényl-N'-phénylaceto-hydrazide and determination of binding parameters with superoxide anion radicals and 2,2-diphenyl-1-picrylhydrazyl

• Main Authors: H. MOUADA, T. LANEZ
• Format: Article
• Language: English
• Published: Phytochemistry & Organic Synthesis Laboratory 2019-03-01
• Series: PhytoChem & BioSub Journal
• Online Access: https://drive.google.com/file/d/1uySqC_HKM0rIJPO0fMzVyIL-iAqhEKhh/view?usp=sharing

The antioxidant activity of N’-méthylferrocéyl-N'-phénylaceto-hydrazide was surveyed utilizing spectrophotometrical and electrochemical technics. We used 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) for the Spectrophotometrical measures and the cyclic voltammetry assays were supported activity by measuring the reaction peak current of comparatively stable superoxide (O2°- ) electrochemically produced by reduction of commercial molecular oxygen in DMF. The interaction of ferrocene derivative N'-ferrocenylmethyl-N’- phenylacetohydrazide with DPPH and O2°-appears good radicals scavenging activities (0.03787 and 1.22 mg/ml) severally that was considerably nearer to that of standard antioxidant ascorbic acid. The diffusion coefficients of the free and O2°-bound forms of N’-ferrocenylmethyl-N’-phenylacetohydrazide were assessed from Randles-Sevcik equation. Cyclic Voltammetry based tests were created for the calculation of binding parameters like binding constant, the proportion of binding constants and binding free energy.