Antioxidant capacity of N’-méthylferrocényl-N'-phénylaceto-hydrazide and determination of binding parameters with superoxide anion radicals and 2,2-diphenyl-1-picrylhydrazyl
The antioxidant activity of N’-méthylferrocéyl-N'-phénylaceto-hydrazide was surveyed utilizing spectrophotometrical and electrochemical technics. We used 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) for the Spectrophotometrical measures and the cyclic voltammetry assays were supported activit...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Phytochemistry & Organic Synthesis Laboratory
2019-03-01
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| Series: | PhytoChem & BioSub Journal |
| Online Access: | https://drive.google.com/file/d/1uySqC_HKM0rIJPO0fMzVyIL-iAqhEKhh/view?usp=sharing |
| Summary: | The antioxidant activity of N’-méthylferrocéyl-N'-phénylaceto-hydrazide was surveyed utilizing
spectrophotometrical and electrochemical technics. We used 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) for
the Spectrophotometrical measures and the cyclic voltammetry assays were supported activity by measuring the
reaction peak current of comparatively stable superoxide (O2°- ) electrochemically produced by reduction of
commercial molecular oxygen in DMF. The interaction of ferrocene derivative N'-ferrocenylmethyl-N’-
phenylacetohydrazide with DPPH and O2°-appears good radicals scavenging activities (0.03787 and 1.22
mg/ml) severally that was considerably nearer to that of standard antioxidant ascorbic acid. The diffusion
coefficients of the free and O2°-bound forms of N’-ferrocenylmethyl-N’-phenylacetohydrazide were assessed from
Randles-Sevcik equation. Cyclic Voltammetry based tests were created for the calculation of binding parameters
like binding constant, the proportion of binding constants and binding free energy.
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| ISSN: | 2170-1768 2170-1768 |