Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR
<p>Abstract</p> <p>Although PCR and RT-PCR provided a valuable approach for detection of pathogens, the high level of sensitivity of these assays also makes them prone to false positive results. In addition to cross-contamination with true positive samples, false positive results a...
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Format: | Article |
Language: | English |
Published: |
BMC
2005-04-01
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Series: | Virology Journal |
Online Access: | http://www.virologyj.com/content/2/1/29 |