Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences

Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences

Two Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin...

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Main Authors: Veli-Matti Rokka, Nora L. V. Lapitan, Dennis L. Knudson, Eija Pehu
Format: Article
Language:English
Published: Scientific Agricultural Society of Finland 1998-01-01
Series:Agricultural and Food Science
Online Access:https://journal.fi/afs/article/view/72853
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spelling doaj-529025325eb44d25a2420a7d6f6c47ff2020-11-24T23:22:55ZengScientific Agricultural Society of FinlandAgricultural and Food Science1459-60671795-18951998-01-0171Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequencesVeli-Matti Rokka0Nora L. V. Lapitan1Dennis L. Knudson2Eija Pehu3Agricultural Research Centre of Finland, Plant Production Research, Crops and Soil, FIN-31600 Jokioinen, FinlandDepartment of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USADepartment of Plant Production, PO Box 27, FIN-00014 University of Helsinki, FinlandTwo Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin-14-dATP and they were detected with reporter molecules conjugated to fluorescent dyes using digital imaging. The tandemly repeated sequences hybridized mostly near the telomeres of the chromosomes of S. brevidens. Using these two probes, it was possible to identify chromosomes containing repetitive DNA of S. brevidens both in the somatic hybrids between S. brevidens and S. tuberosum, and somatohaploids derived from the somatic hybrids. These cytological analyses showed that for the largest part genomes of the hexaploid somatic hybrids and their anther-derived triploid somatohaploids were composed of the genome of S. brevidens.https://journal.fi/afs/article/view/72853
collection DOAJ
language English
format Article
sources DOAJ
author Veli-Matti Rokka
Nora L. V. Lapitan
Dennis L. Knudson
Eija Pehu
spellingShingle Veli-Matti Rokka
Nora L. V. Lapitan
Dennis L. Knudson
Eija Pehu
Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
Agricultural and Food Science
author_facet Veli-Matti Rokka
Nora L. V. Lapitan
Dennis L. Knudson
Eija Pehu
author_sort Veli-Matti Rokka
title Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
title_short Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
title_full Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
title_fullStr Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
title_full_unstemmed Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
title_sort fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two solanum brevidens specific sequences
publisher Scientific Agricultural Society of Finland
series Agricultural and Food Science
issn 1459-6067
1795-1895
publishDate 1998-01-01
description Two Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin-14-dATP and they were detected with reporter molecules conjugated to fluorescent dyes using digital imaging. The tandemly repeated sequences hybridized mostly near the telomeres of the chromosomes of S. brevidens. Using these two probes, it was possible to identify chromosomes containing repetitive DNA of S. brevidens both in the somatic hybrids between S. brevidens and S. tuberosum, and somatohaploids derived from the somatic hybrids. These cytological analyses showed that for the largest part genomes of the hexaploid somatic hybrids and their anther-derived triploid somatohaploids were composed of the genome of S. brevidens.
url https://journal.fi/afs/article/view/72853
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AT noralvlapitan fluorescenceinsituhybridizationofpotatosomatohaploidsandtheirsomatichybriddonorsusingtwosolanumbrevidensspecificsequences
AT dennislknudson fluorescenceinsituhybridizationofpotatosomatohaploidsandtheirsomatichybriddonorsusingtwosolanumbrevidensspecificsequences
AT eijapehu fluorescenceinsituhybridizationofpotatosomatohaploidsandtheirsomatichybriddonorsusingtwosolanumbrevidensspecificsequences
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