Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.

Among the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of...

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Main Authors: Zhaohai Zhang, Jie Sun, Lihong Hao, Chunqing Liu, Hongye Ma, Li Jia
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3688732?pdf=render
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spelling doaj-5243f83f4da745e38cb3e43a48dc0d952020-11-25T01:28:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6521810.1371/journal.pone.0065218Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.Zhaohai ZhangJie SunLihong HaoChunqing LiuHongye MaLi JiaAmong the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of glycosylation mediated the invasive properties of Hca-F and Hca-P murine hepatocarcinoma cell lines, which have high, low metastatic potential in the lymph nodes, respectively. Analysis revealed that the N-glycan composition profiling, expression of glycogenes and lectin binding profiling were different in Hca-F cells, as compared to those in Hca-P cells. Further analysis of the N-glycan regulation by tunicamycin (TM) application or PNGase F treatment in Hca-F cells showed partial inhibition of N-glycan glycosylation and decreased invasion both in vitro and in vivo. We targeted glycogene ST6GAL1, which was expressed differently in Hca-F and Hca-P cells, and regulated the expression of ST6GAL1. The altered levels of ST6GAL1 were also responsible for changed invasive properties of Hca-F and Hca-P cells both in vitro and in vivo. These findings indicate a role for glycosylation modification as a mediator of tumor lymphatic metastasis, with its altered expression causing an invasive ability differentially.http://europepmc.org/articles/PMC3688732?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Zhaohai Zhang
Jie Sun
Lihong Hao
Chunqing Liu
Hongye Ma
Li Jia
spellingShingle Zhaohai Zhang
Jie Sun
Lihong Hao
Chunqing Liu
Hongye Ma
Li Jia
Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
PLoS ONE
author_facet Zhaohai Zhang
Jie Sun
Lihong Hao
Chunqing Liu
Hongye Ma
Li Jia
author_sort Zhaohai Zhang
title Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
title_short Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
title_full Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
title_fullStr Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
title_full_unstemmed Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
title_sort modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Among the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of glycosylation mediated the invasive properties of Hca-F and Hca-P murine hepatocarcinoma cell lines, which have high, low metastatic potential in the lymph nodes, respectively. Analysis revealed that the N-glycan composition profiling, expression of glycogenes and lectin binding profiling were different in Hca-F cells, as compared to those in Hca-P cells. Further analysis of the N-glycan regulation by tunicamycin (TM) application or PNGase F treatment in Hca-F cells showed partial inhibition of N-glycan glycosylation and decreased invasion both in vitro and in vivo. We targeted glycogene ST6GAL1, which was expressed differently in Hca-F and Hca-P cells, and regulated the expression of ST6GAL1. The altered levels of ST6GAL1 were also responsible for changed invasive properties of Hca-F and Hca-P cells both in vitro and in vivo. These findings indicate a role for glycosylation modification as a mediator of tumor lymphatic metastasis, with its altered expression causing an invasive ability differentially.
url http://europepmc.org/articles/PMC3688732?pdf=render
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