A simple and non-invasive method for nuclear transformation of intact-walled Chlamydomonas reinhardtii.

Genetic engineering in microalgae is gaining attraction but nuclear transformation methods available so far are either inefficient or require special equipment. In this study, we employ positively charged nanoparticles, 3-aminopropyl-functionalized magnesium phyllosilicate (aminoclay, approximate un...

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Bibliographic Details
Main Authors: Sora Kim, Young-Chul Lee, Dae-Hyun Cho, Hyun Uk Lee, Yun Suk Huh, Geun-Joong Kim, Hee-Sik Kim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4079685?pdf=render