Alpha-amylase Immobilization on Modified Polyimide Material
In this study, a-amylase was covalently immobilized on modified polyimide materials. Polyimide (PI) was prepared with pyromellitic dianhydride (PMDA) and 4,4’-oxydianline (4,4’-ODA) in the solution of N,N- dimethylformamide (DMF). Free amine groups on the surface of the polyimide membranes were gene...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIDIC Servizi S.r.l.
2013-06-01
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| Series: | Chemical Engineering Transactions |
| Online Access: | https://www.cetjournal.it/index.php/cet/article/view/6676 |
| Summary: | In this study, a-amylase was covalently immobilized on modified polyimide materials. Polyimide (PI) was prepared with pyromellitic dianhydride (PMDA) and 4,4’-oxydianline (4,4’-ODA) in the solution of N,N- dimethylformamide (DMF). Free amine groups on the surface of the polyimide membranes were generated by the amination reaction of polyimides with hexamethylenediamine (HMDA). Surface-aminated membranes were then subjected to enzyme immobilization. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM). Chemical structure of PMDA-ODA PI membranes was characterized by FTIR. SEM and FTIR results showed that the enzyme was successfully covalently attached to the polymeric support. Immobilization efficiency and enzyme activity of a-amylase was examined at various pH values (3.0 – 8.0) and temperatures (15 – 80 °C). Immobilization yield was found to be 285.45 mg per gram for the modified polyimide films. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermo-stability than the free one. |
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| ISSN: | 2283-9216 |