Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway
Abstract Changes in illumination can rapidly influence behavior that is normally controlled by the circadian clock. This effect is termed masking. In mice, masking requires melanopsin-expressing retinal ganglion cells that detect blue light and project to the thalamus. It is not known whether maskin...
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2017-06-01
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Online Access: | https://doi.org/10.1038/s41598-017-04205-7 |
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doaj-5213b7cb3e484115b2ce66593b8c4ac52020-12-08T02:40:37ZengNature Publishing GroupScientific Reports2045-23222017-06-017111110.1038/s41598-017-04205-7Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathwayQian Lin0Suresh Jesuthasan1NUS Graduate School for Integrative Sciences and Engineering, 28 Medical Drive, National University of SingaporeLee Kong Chian School of Medicine, Nanyang Technological UniversityAbstract Changes in illumination can rapidly influence behavior that is normally controlled by the circadian clock. This effect is termed masking. In mice, masking requires melanopsin-expressing retinal ganglion cells that detect blue light and project to the thalamus. It is not known whether masking is wavelength-dependent in other vertebrates, nor is it known whether the thalamus is also involved or how it influences masking. Here, we address these questions in zebrafish. We find that diel vertical migration, a circadian behavior in larval zebrafish, is effectively triggered by blue, but not by red light. Two-photon calcium imaging reveals that a thalamic nucleus and a downstream structure, the habenula, have a sustained response to blue but not to red light. Lesioning the habenula reduces light-evoked climbing. These data suggest that the thalamo-habenula pathway is involved in the ability of blue light to influence a circadian behavior.https://doi.org/10.1038/s41598-017-04205-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qian Lin Suresh Jesuthasan |
spellingShingle |
Qian Lin Suresh Jesuthasan Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway Scientific Reports |
author_facet |
Qian Lin Suresh Jesuthasan |
author_sort |
Qian Lin |
title |
Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway |
title_short |
Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway |
title_full |
Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway |
title_fullStr |
Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway |
title_full_unstemmed |
Masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway |
title_sort |
masking of a circadian behavior in larval zebrafish involves the thalamo-habenula pathway |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Abstract Changes in illumination can rapidly influence behavior that is normally controlled by the circadian clock. This effect is termed masking. In mice, masking requires melanopsin-expressing retinal ganglion cells that detect blue light and project to the thalamus. It is not known whether masking is wavelength-dependent in other vertebrates, nor is it known whether the thalamus is also involved or how it influences masking. Here, we address these questions in zebrafish. We find that diel vertical migration, a circadian behavior in larval zebrafish, is effectively triggered by blue, but not by red light. Two-photon calcium imaging reveals that a thalamic nucleus and a downstream structure, the habenula, have a sustained response to blue but not to red light. Lesioning the habenula reduces light-evoked climbing. These data suggest that the thalamo-habenula pathway is involved in the ability of blue light to influence a circadian behavior. |
url |
https://doi.org/10.1038/s41598-017-04205-7 |
work_keys_str_mv |
AT qianlin maskingofacircadianbehaviorinlarvalzebrafishinvolvesthethalamohabenulapathway AT sureshjesuthasan maskingofacircadianbehaviorinlarvalzebrafishinvolvesthethalamohabenulapathway |
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