Methods of extraction and thin-layer chromatography determination of phospholipids in biological samples

Phospholipids (PLs) are a class of compounds essential for living organisms, including human beings. This paper presents a brief review concerning the extraction as well as thin-layer chromatographic separation and determination of phospholipids. After a short introduction regarding phospholipids an...

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Bibliographic Details
Main Authors: Dyńska-Kukulska Katarzyna, Ciesielski Witold
Format: Article
Language:English
Published: De Gruyter 2012-03-01
Series:Reviews in Analytical Chemistry
Subjects:
Online Access:https://doi.org/10.1515/revac-2011-0030
Description
Summary:Phospholipids (PLs) are a class of compounds essential for living organisms, including human beings. This paper presents a brief review concerning the extraction as well as thin-layer chromatographic separation and determination of phospholipids. After a short introduction regarding phospholipids and their specific characteristics, the extraction techniques will be discussed. Then, phospholipid separation and system detection for thin-layer chromatography (TLC) will be presented. The focus will be on phospholipids that are most abundant in biological systems; that is why chosen examples of PLs determination in biological samples will be revealed as well as general PL determination schemes. In the Summary section, there will also be a short discussion about advantages and disadvantages of PL determination with the use of TLC.
ISSN:0793-0135
2191-0189