Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells

<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with i...

Full description

Bibliographic Details
Main Authors: Hua Zhang, Nan Cai, Min Feng, Juan Zhu, Wei Xu, Qing Feng, Zheng Liu
Format: Article
Language:English
Published: BMC 2011-07-01
Series:Journal of Experimental & Clinical Cancer Research
Online Access:http://www.jeccr.com/content/30/1/67
id doaj-51742b1155884df5b6efdb02dd23348c
record_format Article
spelling doaj-51742b1155884df5b6efdb02dd23348c2020-11-24T21:23:49ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662011-07-013016710.1186/1756-9966-30-67Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cellsHua ZhangNan CaiMin FengJuan ZhuWei XuQing FengZheng Liu<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [<sup>3</sup>H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.</p> <p>Results</p> <p>Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.</p> <p>Conclusions</p> <p>IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines <it>in vitro</it>, and it provides an experimental basis for c-Met-targeted therapy towards <it>in vivo </it>testing.</p> http://www.jeccr.com/content/30/1/67
collection DOAJ
language English
format Article
sources DOAJ
author Hua Zhang
Nan Cai
Min Feng
Juan Zhu
Wei Xu
Qing Feng
Zheng Liu
spellingShingle Hua Zhang
Nan Cai
Min Feng
Juan Zhu
Wei Xu
Qing Feng
Zheng Liu
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
Journal of Experimental & Clinical Cancer Research
author_facet Hua Zhang
Nan Cai
Min Feng
Juan Zhu
Wei Xu
Qing Feng
Zheng Liu
author_sort Hua Zhang
title Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
title_short Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
title_full Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
title_fullStr Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
title_full_unstemmed Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
title_sort recombinant immunotoxin anti-c-met/pe38kdel inhibits proliferation and promotes apoptosis of gastric cancer cells
publisher BMC
series Journal of Experimental & Clinical Cancer Research
issn 1756-9966
publishDate 2011-07-01
description <p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [<sup>3</sup>H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.</p> <p>Results</p> <p>Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.</p> <p>Conclusions</p> <p>IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines <it>in vitro</it>, and it provides an experimental basis for c-Met-targeted therapy towards <it>in vivo </it>testing.</p>
url http://www.jeccr.com/content/30/1/67
work_keys_str_mv AT huazhang recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
AT nancai recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
AT minfeng recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
AT juanzhu recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
AT weixu recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
AT qingfeng recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
AT zhengliu recombinantimmunotoxinanticmetpe38kdelinhibitsproliferationandpromotesapoptosisofgastriccancercells
_version_ 1725991128230002688