Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with i...
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doaj-51742b1155884df5b6efdb02dd23348c2020-11-24T21:23:49ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662011-07-013016710.1186/1756-9966-30-67Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cellsHua ZhangNan CaiMin FengJuan ZhuWei XuQing FengZheng Liu<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [<sup>3</sup>H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.</p> <p>Results</p> <p>Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.</p> <p>Conclusions</p> <p>IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines <it>in vitro</it>, and it provides an experimental basis for c-Met-targeted therapy towards <it>in vivo </it>testing.</p> http://www.jeccr.com/content/30/1/67 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua Zhang Nan Cai Min Feng Juan Zhu Wei Xu Qing Feng Zheng Liu |
spellingShingle |
Hua Zhang Nan Cai Min Feng Juan Zhu Wei Xu Qing Feng Zheng Liu Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells Journal of Experimental & Clinical Cancer Research |
author_facet |
Hua Zhang Nan Cai Min Feng Juan Zhu Wei Xu Qing Feng Zheng Liu |
author_sort |
Hua Zhang |
title |
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells |
title_short |
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells |
title_full |
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells |
title_fullStr |
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells |
title_full_unstemmed |
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells |
title_sort |
recombinant immunotoxin anti-c-met/pe38kdel inhibits proliferation and promotes apoptosis of gastric cancer cells |
publisher |
BMC |
series |
Journal of Experimental & Clinical Cancer Research |
issn |
1756-9966 |
publishDate |
2011-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [<sup>3</sup>H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.</p> <p>Results</p> <p>Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.</p> <p>Conclusions</p> <p>IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines <it>in vitro</it>, and it provides an experimental basis for c-Met-targeted therapy towards <it>in vivo </it>testing.</p> |
url |
http://www.jeccr.com/content/30/1/67 |
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