Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

Optimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.

Bibliographic Details
Main Authors:	Xin Chen, Na Liu, Liquan Liu, Wei Chen, Na Chen, Meng Lin, Jiaju Xu, Xing Zhou, Hongbo Wang, Meiping Zhao, Xianjin Xiao
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-10-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-12593-9

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spelling	doaj-51692b9b0b4546f3a6c6b9e302c3c6832021-05-11T12:20:44ZengNature Publishing GroupNature Communications2041-17232019-10-0110111210.1038/s41467-019-12593-9Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimizationXin Chen0Na Liu1Liquan Liu2Wei Chen3Na Chen4Meng Lin5Jiaju Xu6Xing Zhou7Hongbo Wang8Meiping Zhao9Xianjin Xiao10Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyBeijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking UniversityInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyBeijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking UniversityInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyOptimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.https://doi.org/10.1038/s41467-019-12593-9
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xin Chen Na Liu Liquan Liu Wei Chen Na Chen Meng Lin Jiaju Xu Xing Zhou Hongbo Wang Meiping Zhao Xianjin Xiao
spellingShingle	Xin Chen Na Liu Liquan Liu Wei Chen Na Chen Meng Lin Jiaju Xu Xing Zhou Hongbo Wang Meiping Zhao Xianjin Xiao Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization Nature Communications
author_facet	Xin Chen Na Liu Liquan Liu Wei Chen Na Chen Meng Lin Jiaju Xu Xing Zhou Hongbo Wang Meiping Zhao Xianjin Xiao
author_sort	Xin Chen
title	Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization
title_short	Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization
title_full	Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization
title_fullStr	Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization
title_full_unstemmed	Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization
title_sort	thermodynamics and kinetics guided probe design for uniformly sensitive and specific dna hybridization without optimization
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-10-01
description	Optimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.
url	https://doi.org/10.1038/s41467-019-12593-9
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Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

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