Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring

Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practicall...

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Main Authors:	Robert Robinson, Liam Wong, Raymond J. Monnat, Elain Fu
Format:	Article
Language:	English
Published:	MDPI AG 2016-02-01
Series:	Micromachines
Subjects:	paper-based device whole-blood assay phenylalanine detection colorimetric readout phenylketonuria
Online Access:	http://www.mdpi.com/2072-666X/7/2/28

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spelling	doaj-51606a63f60e46c5abf5d9838a73200e2020-11-24T22:23:07ZengMDPI AGMicromachines2072-666X2016-02-01722810.3390/mi7020028mi7020028Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy MonitoringRobert Robinson0Liam Wong1Raymond J. Monnat2Elain Fu3School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USASchool of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USADepartments of Pathology and Genome Sciences, University of Washington, Seattle, WA 98195, USASchool of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USALaboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 μL) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients.http://www.mdpi.com/2072-666X/7/2/28paper-based devicewhole-blood assayphenylalanine detectioncolorimetric readoutphenylketonuria
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Robert Robinson Liam Wong Raymond J. Monnat Elain Fu
spellingShingle	Robert Robinson Liam Wong Raymond J. Monnat Elain Fu Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring Micromachines paper-based device whole-blood assay phenylalanine detection colorimetric readout phenylketonuria
author_facet	Robert Robinson Liam Wong Raymond J. Monnat Elain Fu
author_sort	Robert Robinson
title	Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_short	Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_full	Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_fullStr	Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_full_unstemmed	Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_sort	development of a whole blood paper-based device for phenylalanine detection in the context of pku therapy monitoring
publisher	MDPI AG
series	Micromachines
issn	2072-666X
publishDate	2016-02-01
description	Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 μL) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients.
topic	paper-based device whole-blood assay phenylalanine detection colorimetric readout phenylketonuria
url	http://www.mdpi.com/2072-666X/7/2/28
work_keys_str_mv	AT robertrobinson developmentofawholebloodpaperbaseddeviceforphenylalaninedetectioninthecontextofpkutherapymonitoring AT liamwong developmentofawholebloodpaperbaseddeviceforphenylalaninedetectioninthecontextofpkutherapymonitoring AT raymondjmonnat developmentofawholebloodpaperbaseddeviceforphenylalaninedetectioninthecontextofpkutherapymonitoring AT elainfu developmentofawholebloodpaperbaseddeviceforphenylalaninedetectioninthecontextofpkutherapymonitoring
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Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring

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