Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation

Abstract Background Although typically cultured at an atmospheric oxygen concentration (20–21%), adipose-derived stem cells (ASCs) reside under considerable low oxygen tension (physioxia) in vivo. In the present study, we explored whether and how physioxia could be a more effective strategy for cult...

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Main Authors: Chang Chen, Qi Tang, Yan Zhang, Mei Yu, Wei Jing, Weidong Tian
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Stem Cell Research & Therapy
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13287-018-0891-4
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spelling doaj-5141b2e5adc34ccc8b914b37483b85f62020-11-24T20:53:36ZengBMCStem Cell Research & Therapy1757-65122018-05-019111210.1186/s13287-018-0891-4Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantationChang Chen0Qi Tang1Yan Zhang2Mei Yu3Wei Jing4Weidong Tian5State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityAbstract Background Although typically cultured at an atmospheric oxygen concentration (20–21%), adipose-derived stem cells (ASCs) reside under considerable low oxygen tension (physioxia) in vivo. In the present study, we explored whether and how physioxia could be a more effective strategy for culturing ASCs for transplantation. Methods After isolation, human ASCs were cultured under physioxia (2% O2) and hyperoxia (20% O2) until assayed. WST-8, Transwell, tube formation, β-galactosidase staining, and annexin V-FITC/PI assays were used to evaluate cell proliferation, migration, angiogenesis, senescence, and apoptosis, respectively. Survivability was determined by an ischemia model in vitro and nude mouse model in vivo, and the underlying metabolic alterations were investigated by fluorescence staining, flow cytometry, and real-time polymerase chain reaction. Results Compared with those in the hyperoxia group, cells in the physioxia group exhibited increased proliferation, migration, and angiogenesis, and decreased senescence and apoptosis. The increased survival rate of ASCs cultured in physioxia was found both in ischemia model in vitro and in vivo. The underlying metabolic reprogramming was also monitored and showed decreased mitochondrial mass, alkalized intracellular pH, and increased glucose uptake and glycogen synthesis. Conclusions These results suggest that physioxia is a more effective environment in which to culture ASCs for transplantation owing to the maintenance of native bioactivities without injury by hyperoxia.http://link.springer.com/article/10.1186/s13287-018-0891-4PhysioxiaAdipose-derived stem cellsCell survivalCulture approachCell therapy
collection DOAJ
language English
format Article
sources DOAJ
author Chang Chen
Qi Tang
Yan Zhang
Mei Yu
Wei Jing
Weidong Tian
spellingShingle Chang Chen
Qi Tang
Yan Zhang
Mei Yu
Wei Jing
Weidong Tian
Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
Stem Cell Research & Therapy
Physioxia
Adipose-derived stem cells
Cell survival
Culture approach
Cell therapy
author_facet Chang Chen
Qi Tang
Yan Zhang
Mei Yu
Wei Jing
Weidong Tian
author_sort Chang Chen
title Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
title_short Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
title_full Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
title_fullStr Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
title_full_unstemmed Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
title_sort physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation
publisher BMC
series Stem Cell Research & Therapy
issn 1757-6512
publishDate 2018-05-01
description Abstract Background Although typically cultured at an atmospheric oxygen concentration (20–21%), adipose-derived stem cells (ASCs) reside under considerable low oxygen tension (physioxia) in vivo. In the present study, we explored whether and how physioxia could be a more effective strategy for culturing ASCs for transplantation. Methods After isolation, human ASCs were cultured under physioxia (2% O2) and hyperoxia (20% O2) until assayed. WST-8, Transwell, tube formation, β-galactosidase staining, and annexin V-FITC/PI assays were used to evaluate cell proliferation, migration, angiogenesis, senescence, and apoptosis, respectively. Survivability was determined by an ischemia model in vitro and nude mouse model in vivo, and the underlying metabolic alterations were investigated by fluorescence staining, flow cytometry, and real-time polymerase chain reaction. Results Compared with those in the hyperoxia group, cells in the physioxia group exhibited increased proliferation, migration, and angiogenesis, and decreased senescence and apoptosis. The increased survival rate of ASCs cultured in physioxia was found both in ischemia model in vitro and in vivo. The underlying metabolic reprogramming was also monitored and showed decreased mitochondrial mass, alkalized intracellular pH, and increased glucose uptake and glycogen synthesis. Conclusions These results suggest that physioxia is a more effective environment in which to culture ASCs for transplantation owing to the maintenance of native bioactivities without injury by hyperoxia.
topic Physioxia
Adipose-derived stem cells
Cell survival
Culture approach
Cell therapy
url http://link.springer.com/article/10.1186/s13287-018-0891-4
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