Detection of ligation products of DNA linkers with 5'-OH ends by denaturing PAGE silver stain.

To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases. The ligation products were detected by using de...

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Bibliographic Details
Main Authors: Feng Gao, Huafu Zhou, Wei Li, Xuerong Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3384673?pdf=render

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