The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering

The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering

The growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising m...

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Main Authors: Julia Radwan-Pragłowska, Klaudia Stangel-Wójcikiewicz, Marek Piątkowski, Łukasz Janus, Dalibor Matýsek, Marcin Majka, Dalia Amrom
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Molecules
Subjects:
chitosan
biomaterials
pelvic organ prolapse
Online Access:https://www.mdpi.com/1420-3049/25/18/4280
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spelling doaj-51258b3719964b1b86ada854c5c03fbd2020-11-25T03:32:35ZengMDPI AGMolecules1420-30492020-09-01254280428010.3390/molecules25184280The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue EngineeringJulia Radwan-Pragłowska0Klaudia Stangel-Wójcikiewicz1Marek Piątkowski2Łukasz Janus3Dalibor Matýsek4Marcin Majka5Dalia Amrom6Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandGynecology and Oncology Department Jagiellonian University Collegium Medicum, Kopernika 23, 31-501 Kraków, PolandDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandFaculty of Mining and Geology, Technical University of Ostrava, 708 00 Ostrava, Czech RepublicTransplantology Department Jagiellonian University Collegium Medicum, Wielicka 265, 30-663 Kraków, PolandGynecology and Oncology Department Jagiellonian University Collegium Medicum, Kopernika 23, 31-501 Kraków, PolandThe growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising materials is chitosan, which is known for its biocompatibility and biodegradability. The aim of the following study was to verify the potential of chitosan-based biomaterials for pelvic organ prolapse regeneration. The scaffolds were obtained under microwave-assisted conditions in crosslinking reactions, using dicarboxylic acids and aminoacid as crosslinkers, including <span style="font-variant: small-caps;">l</span>-glutamic acid, adipic acid, malonic acid, and levulinic acid. The products were characterized over their physicochemical and biological properties. FT–IR analysis confirmed formation of amide bonds. The scaffolds had a highly porous structure, which was confirmed by SEM analysis. Their porosity was above 90%. The biomaterials had excellent swelling abilities and very good antioxidant properties. The cytotoxicity study was performed on vaginal epithelial VK2/E6E7 and human colon cancer HCT116 cell lines. The results showed that after certain modifications, the proposed scaffolds could be used in pelvic organ prolapse (POP) treatment.https://www.mdpi.com/1420-3049/25/18/4280chitosanbiomaterialspelvic organ prolapse
collection DOAJ
language English
format Article
sources DOAJ
author Julia Radwan-Pragłowska
Klaudia Stangel-Wójcikiewicz
Marek Piątkowski
Łukasz Janus
Dalibor Matýsek
Marcin Majka
Dalia Amrom
spellingShingle Julia Radwan-Pragłowska
Klaudia Stangel-Wójcikiewicz
Marek Piątkowski
Łukasz Janus
Dalibor Matýsek
Marcin Majka
Dalia Amrom
The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering
Molecules
chitosan
biomaterials
pelvic organ prolapse
author_facet Julia Radwan-Pragłowska
Klaudia Stangel-Wójcikiewicz
Marek Piątkowski
Łukasz Janus
Dalibor Matýsek
Marcin Majka
Dalia Amrom
author_sort Julia Radwan-Pragłowska
title The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering
title_short The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering
title_full The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering
title_fullStr The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering
title_full_unstemmed The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering
title_sort potential of novel chitosan-based scaffolds in pelvic organ prolapse (pop) treatment through tissue engineering
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2020-09-01
description The growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising materials is chitosan, which is known for its biocompatibility and biodegradability. The aim of the following study was to verify the potential of chitosan-based biomaterials for pelvic organ prolapse regeneration. The scaffolds were obtained under microwave-assisted conditions in crosslinking reactions, using dicarboxylic acids and aminoacid as crosslinkers, including <span style="font-variant: small-caps;">l</span>-glutamic acid, adipic acid, malonic acid, and levulinic acid. The products were characterized over their physicochemical and biological properties. FT–IR analysis confirmed formation of amide bonds. The scaffolds had a highly porous structure, which was confirmed by SEM analysis. Their porosity was above 90%. The biomaterials had excellent swelling abilities and very good antioxidant properties. The cytotoxicity study was performed on vaginal epithelial VK2/E6E7 and human colon cancer HCT116 cell lines. The results showed that after certain modifications, the proposed scaffolds could be used in pelvic organ prolapse (POP) treatment.
topic chitosan
biomaterials
pelvic organ prolapse
url https://www.mdpi.com/1420-3049/25/18/4280
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