Inhibitory Effect of Cinnamaldehyde on Main Destructive Microorganisms of Nanhai No. 1 Shipwreck

• Main Authors: Xinduo Huang, Yeqing Han, Jing Du, Peifeng Guo, Yu Wang, Kaixuan Ma, Naisheng Li, Zhiguo Zhang, Yue Li, Jiao Pan
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Applied Sciences
• Subjects: Nanhai No. 1 shipwreck; cinnamaldehyde; antimicrobial activity; biodegradation
• Online Access: https://www.mdpi.com/2076-3417/11/11/5262

Nanhai No. 1, a shipwreck in the Southern Song Dynasty, China, has a history of more than 800 years. It was salvaged in 2007 and is now on display in the Guangdong Maritime Silk Road Museum. Due to the fact that the hull is a wooden cultural relic and exposed to the air, the biological corrosion and biodegradation caused by microorganisms are key problems of hull protection. At present, the antimicrobial agent Euxyl^® K100 (isothiazolinone) has a significant antimicrobial effect in the field, but it has a certain negative impact on the environment and archeologists. In order to reduce the use of chemical antimicrobial agents, we evaluated the inhibitory effects of cinnamaldehyde on the main destructive microorganisms of Nanhai No. 1. Cinnamaldehyde is the main active component of cinnamon, and has broad-spectrum antimicrobial properties. The paper diffusion method, gas diffusion method and minimum inhibitory concentration experiment were used to detect the inhibitory effects of cinnamaldehyde on the main microorganisms of Nanhai No. 1. We found that cinnamaldehyde had significant inhibitory effects on <i>Bacillus tequilensis</i> NK-NH5, <i>Bacillus megaterium</i> NK-NH10, <i>Bacillus velezensis</i> NK-NH11, <i>Bacillus</i> sp. NK-NH15, <i>Bacillus</i> sp. NK-NH16, <i>Bacillus</i> sp. NK-NH17, <i>Fusarium</i><i>solani</i> NK-NH1 and <i>Scedosporium</i><i>apiospermum</i> NK.W1-3. At the same time, cinnamaldehyde had more inhibitory effects on fungi than bacteria. Finally, we verified that cinnamaldehyde can effectively inhibit the growth of microorganisms in water, for storing the scattered wood blocks of the Nanhai No. 1 hull through laboratory simulation experiments. Cinnamaldehyde, as an environment-friendly antimicrobial agent, is of great significance to protecting water-saturated wooden relics from microbial corrosion and degradation in the future.