Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis
Coccidiosis is a major hazard to the chicken industry, but the host’s immune response to coccidiosis remains unclear. Here, we performed Eimeria coccidia challenge in 28-day-old ROSS 308 broilers and selected the bursa from the three most severely affected individuals and three healthy individuals f...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2020.573676/full |
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doaj-510fb719429c40db87add0332541e8a8 |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lijin Guo Lijin Guo Lijin Guo Weiling Huang Weiling Huang Weiling Huang Feng Tong Feng Tong Xiaolan Chen Xiaolan Chen Xiaolan Chen Sen Cao Sen Cao Sen Cao Haiping Xu Haiping Xu Haiping Xu Wei Luo Wei Luo Wei Luo Zhenhui Li Zhenhui Li Zhenhui Li Qinghua Nie Qinghua Nie Qinghua Nie |
| spellingShingle |
Lijin Guo Lijin Guo Lijin Guo Weiling Huang Weiling Huang Weiling Huang Feng Tong Feng Tong Xiaolan Chen Xiaolan Chen Xiaolan Chen Sen Cao Sen Cao Sen Cao Haiping Xu Haiping Xu Haiping Xu Wei Luo Wei Luo Wei Luo Zhenhui Li Zhenhui Li Zhenhui Li Qinghua Nie Qinghua Nie Qinghua Nie Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis Frontiers in Physiology coccidiosis RNA-seq TNFRSF6B macrophages polarization macrophages apoptosis Fas signal pathway |
| author_facet |
Lijin Guo Lijin Guo Lijin Guo Weiling Huang Weiling Huang Weiling Huang Feng Tong Feng Tong Xiaolan Chen Xiaolan Chen Xiaolan Chen Sen Cao Sen Cao Sen Cao Haiping Xu Haiping Xu Haiping Xu Wei Luo Wei Luo Wei Luo Zhenhui Li Zhenhui Li Zhenhui Li Qinghua Nie Qinghua Nie Qinghua Nie |
| author_sort |
Lijin Guo |
| title |
Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis |
| title_short |
Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis |
| title_full |
Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis |
| title_fullStr |
Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis |
| title_full_unstemmed |
Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis |
| title_sort |
whole transcriptome analysis of chicken bursa reveals candidate gene that enhances the host’s immune response to coccidiosis |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Physiology |
| issn |
1664-042X |
| publishDate |
2020-10-01 |
| description |
Coccidiosis is a major hazard to the chicken industry, but the host’s immune response to coccidiosis remains unclear. Here, we performed Eimeria coccidia challenge in 28-day-old ROSS 308 broilers and selected the bursa from the three most severely affected individuals and three healthy individuals for RNA sequencing. We obtained 347 DEGs from RNA-seq and found that 7 upregulated DEGs were enriched in Cytokine-cytokine receptor interaction pathway. As the DEGs with the highest expression abundance in these 7 genes, TNFRSF6B was speculated to participate in the process of host’s immune response to coccidiosis. It is showed that TNFRSF6B can polarize macrophages to M1 subtype and promote inflammatory cytokines expression. In addition, the expression of TNFRSF6B suppressed HD11 cells apoptosis by downregulating Fas signal pathway. Besides, TNFRSF6B-mediated macrophages immunity activation can be reversed by apoptosis. Overall, our study indicates that TNFRSF6B upregulated in BAE, is capable of aggravating the inflammatory response by inhibiting macrophages apoptosis via downregulating Fas signal pathway, which may participate in host’s immune response to coccidiosis. |
| topic |
coccidiosis RNA-seq TNFRSF6B macrophages polarization macrophages apoptosis Fas signal pathway |
| url |
https://www.frontiersin.org/articles/10.3389/fphys.2020.573676/full |
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doaj-510fb719429c40db87add0332541e8a82020-11-25T04:06:12ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-10-011110.3389/fphys.2020.573676573676Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to CoccidiosisLijin Guo0Lijin Guo1Lijin Guo2Weiling Huang3Weiling Huang4Weiling Huang5Feng Tong6Feng Tong7Xiaolan Chen8Xiaolan Chen9Xiaolan Chen10Sen Cao11Sen Cao12Sen Cao13Haiping Xu14Haiping Xu15Haiping Xu16Wei Luo17Wei Luo18Wei Luo19Zhenhui Li20Zhenhui Li21Zhenhui Li22Qinghua Nie23Qinghua Nie24Qinghua Nie25Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, ChinaCoccidiosis is a major hazard to the chicken industry, but the host’s immune response to coccidiosis remains unclear. Here, we performed Eimeria coccidia challenge in 28-day-old ROSS 308 broilers and selected the bursa from the three most severely affected individuals and three healthy individuals for RNA sequencing. We obtained 347 DEGs from RNA-seq and found that 7 upregulated DEGs were enriched in Cytokine-cytokine receptor interaction pathway. As the DEGs with the highest expression abundance in these 7 genes, TNFRSF6B was speculated to participate in the process of host’s immune response to coccidiosis. It is showed that TNFRSF6B can polarize macrophages to M1 subtype and promote inflammatory cytokines expression. In addition, the expression of TNFRSF6B suppressed HD11 cells apoptosis by downregulating Fas signal pathway. Besides, TNFRSF6B-mediated macrophages immunity activation can be reversed by apoptosis. Overall, our study indicates that TNFRSF6B upregulated in BAE, is capable of aggravating the inflammatory response by inhibiting macrophages apoptosis via downregulating Fas signal pathway, which may participate in host’s immune response to coccidiosis.https://www.frontiersin.org/articles/10.3389/fphys.2020.573676/fullcoccidiosisRNA-seqTNFRSF6Bmacrophages polarizationmacrophages apoptosisFas signal pathway |