Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging

Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging

Abstract Background Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level. Results We report the use of multimodal Ra...

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Bibliographic Details
Main Authors: Sadie J. Burkhow, Nicole M. Stephens, Yu Mei, Maria Emilia Dueñas, Daniel J. Freppon, Geng Ding, Shea C. Smith, Young-Jin Lee, Basil J. Nikolau, Steven A. Whitham, Emily A. Smith
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Plant Methods
Subjects:
RNA silencing
Foxtail mosaic virus
Phytoene desaturase
Subcellular Raman imaging
Mass spectrometry imaging
Whole-plant analysis
Online Access:http://link.springer.com/article/10.1186/s13007-018-0306-7

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http://link.springer.com/article/10.1186/s13007-018-0306-7

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