Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis

AIM: To investigate the expression of TWIK-related arachidonic acid-stimulated K+ channel (TRAAK) in retinal degeneration mice (rd1) and further evaluate how TRAAK affect photoreceptor cell apoptosis. METHODS: The rd1 mice were distributed into blank (no treatment), control (1.4% DMSO, intraperiton...

Full description

Bibliographic Details
Main Authors: Lei Wang, Kang-Pei Shi, Han Li, Hao Huang, Wen-Bin Wu, Chu-Sheng Cai, Xiao-Tong Zhang, Xiao-Bo Zhu
Format: Article
Language:English
Published: Press of International Journal of Ophthalmology (IJO PRESS) 2019-08-01
Series:International Journal of Ophthalmology
Subjects:
Online Access:http://www.ijo.cn/en_publish/2019/8/20190803.pdf
id doaj-50f195dc677e4e37bd500e92e819e784
record_format Article
spelling doaj-50f195dc677e4e37bd500e92e819e7842020-11-25T01:28:33ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982019-08-011281243124910.18240/ijo.2019.08.03Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosisLei Wang0Kang-Pei Shi1Han Li2Hao Huang3Wen-Bin Wu4Chu-Sheng Cai5Xiao-Tong Zhang6Xiao-Bo Zhu7State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaAIM: To investigate the expression of TWIK-related arachidonic acid-stimulated K+ channel (TRAAK) in retinal degeneration mice (rd1) and further evaluate how TRAAK affect photoreceptor cell apoptosis. METHODS: The rd1 mice were distributed into blank (no treatment), control (1.4% DMSO, intraperitoneal injection) and riluzole groups (4 mg/kg·d, intraperitoneal injection) from postnatal 7d to 10, 14 and 18d; C57 group (no treatment), as age-matched wild-type control. The thickness of the outer nuclear layer (ONL) of retina was detected by paraffin section hematoxylin and eosin staining. The expression of TRAAK and the apoptosis of the ONL cells were detected by immunostaining, Western blotting, and real-time polymerase chain reaction. RESULTS: The channel agonist riluzole activated TRAAK and delayed the apoptosis of photoreceptor cells in ONL layer of rd1 mice. Both at mRNA and protein levels, after riluzole treatment, TRAAK expression was significantly upregulated, when compared with the control and blank group. Then we detected a series of apoptosis related mRNA and protein. The anti-apoptotic factor Bcl-2 downregulated and the pro-apoptotic factors Bax and cleaved-caspase-3 upregulated significantly. CONCLUSION: Riluzole elevates the expression of TRAAK and inhibits the development of apoptosis. Activation of TRAAK may have some potential effects to put off photoreceptor apoptosis.http://www.ijo.cn/en_publish/2019/8/20190803.pdftraakriluzolephotoreceptor cellapoptosis
collection DOAJ
language English
format Article
sources DOAJ
author Lei Wang
Kang-Pei Shi
Han Li
Hao Huang
Wen-Bin Wu
Chu-Sheng Cai
Xiao-Tong Zhang
Xiao-Bo Zhu
spellingShingle Lei Wang
Kang-Pei Shi
Han Li
Hao Huang
Wen-Bin Wu
Chu-Sheng Cai
Xiao-Tong Zhang
Xiao-Bo Zhu
Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
International Journal of Ophthalmology
traak
riluzole
photoreceptor cell
apoptosis
author_facet Lei Wang
Kang-Pei Shi
Han Li
Hao Huang
Wen-Bin Wu
Chu-Sheng Cai
Xiao-Tong Zhang
Xiao-Bo Zhu
author_sort Lei Wang
title Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
title_short Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
title_full Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
title_fullStr Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
title_full_unstemmed Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
title_sort activation of the traak two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis
publisher Press of International Journal of Ophthalmology (IJO PRESS)
series International Journal of Ophthalmology
issn 2222-3959
2227-4898
publishDate 2019-08-01
description AIM: To investigate the expression of TWIK-related arachidonic acid-stimulated K+ channel (TRAAK) in retinal degeneration mice (rd1) and further evaluate how TRAAK affect photoreceptor cell apoptosis. METHODS: The rd1 mice were distributed into blank (no treatment), control (1.4% DMSO, intraperitoneal injection) and riluzole groups (4 mg/kg·d, intraperitoneal injection) from postnatal 7d to 10, 14 and 18d; C57 group (no treatment), as age-matched wild-type control. The thickness of the outer nuclear layer (ONL) of retina was detected by paraffin section hematoxylin and eosin staining. The expression of TRAAK and the apoptosis of the ONL cells were detected by immunostaining, Western blotting, and real-time polymerase chain reaction. RESULTS: The channel agonist riluzole activated TRAAK and delayed the apoptosis of photoreceptor cells in ONL layer of rd1 mice. Both at mRNA and protein levels, after riluzole treatment, TRAAK expression was significantly upregulated, when compared with the control and blank group. Then we detected a series of apoptosis related mRNA and protein. The anti-apoptotic factor Bcl-2 downregulated and the pro-apoptotic factors Bax and cleaved-caspase-3 upregulated significantly. CONCLUSION: Riluzole elevates the expression of TRAAK and inhibits the development of apoptosis. Activation of TRAAK may have some potential effects to put off photoreceptor apoptosis.
topic traak
riluzole
photoreceptor cell
apoptosis
url http://www.ijo.cn/en_publish/2019/8/20190803.pdf
work_keys_str_mv AT leiwang activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT kangpeishi activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT hanli activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT haohuang activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT wenbinwu activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT chushengcai activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT xiaotongzhang activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
AT xiaobozhu activationofthetraaktwoporedomainpotassiumchannelsinrd1miceprotectsphotoreceptorcellsfromapoptosis
_version_ 1725100892714172416