| Summary: | ABSTRACT Equisetum arvense, a fern species possesses a number of pharmaceutical prospective. In the present study, a total of 103 endophytic bacteria isolated from E. arvense and were evaluated for their anticandidal property against five Candida species, two C. albicans, C. glabrata, C. saitoana and C. geochares. Out of them fifty one were identified as per the morphological and molecular characterisation using 16S rRNA gene sequencing and among them, ten promising endophytic bacteria were mentioned in the present study. Among ten endophytic bacteria, Psychrobacillus insolitus and Curtobacterium oceanosedimentum exerted highest anticandidal effect against C. albicans KACC 30062 and C. glabrata KBNO6P00368, with diameter of inhibition zones of 21.30±0.41 and 18.24±0.12 mm, respectively. When the endophytic bacteria cultures were successively fractionated using different solvents, only the butanol fraction of Psychrobacillus insolitus and Curtobacterium oceanosedimentum had anticandidal activity, with inhibition zones of 20.12±0.28 mm and 12.33±0.11 mm, respectively. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of the butanol fractions ranged from 250 to 500 and 500 to 1,000 µg/mL, respectively. Scanning electron microscope (SEM) analysis showed impaired membrane of C. albicans and C. glabrata at the MIC, indicating that butanol extract lysed the cell membrane and caused cell death. The endophytic bacteria derived from E. arvense can be a valuable resource for the development of natural anticandidal agents to manage candidiasis.
|
|---|
